Electrospun protein-CNT composite fibers and the application in fibroblast stimulation
Electrospun protein-CNT composite fibers and the application in fibroblast stimulation
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DOI：
10.1016/j.bbrc.2018.08.157
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发表时间：
2018-09-26
影响因子：
3.1
通讯作者：
Wang, Rong
中科院分区：
文献类型：
--
作者：
Chi, Naiwei;Wang, Rong
功能性生物聚合物支架在组织再生中有很高的需求。在本研究中，我们将功能化碳纳米管掺入胶原蛋白或丝蛋白溶液中，通过静电纺丝制备生物复合纤维。碳纳米管的加入增强了支架的强度，并使纤维具有导电性，不仅促进了e -纺纤维的形成，而且还赋予纤维额外的功能，可用于细胞刺激。考虑到纤维的尺寸、排列、机械强度、导电性和生物相容性，含有微量碳纳米管（0.05%）的丝-碳纳米管纤维优于其他类型的纤维。通过诱导成纤维细胞的极化和细胞缺陷的激活，研究了这些纤维的调节作用。虽然胶原-碳纳米管和丝-碳纳米管纤维上的成纤维细胞比纯蛋白纤维上的细胞合成的III型胶原蛋白（COLIII）水平要高得多，以降低异常高的COLI/COLIII比率，但由于丝-碳纳米管的高导电性，电刺激使丝-碳纳米管上的细胞的胶原蛋白产量比胶原-碳纳米管上的细胞高20倍。所开发的方法可以潜在地用于修复功能失调的成纤维细胞，以治疗与胶原蛋白紊乱相关的疾病和健康状况。(C) 2018爱思唯尔公司版权所有。
Functional biopolymer scaffolds are in high demand for tissue regeneration. In this study, we incorporated functionalized CNT in collagen or silk protein solution to generate biocomposite fibers by electrospinning. The addition of CNT reinforced the strength of the scaffolds and rendered the fibers electrical conductivity to not only facilitate the E-spun fiber formation but also grant the fibers an additional functionality that can be utilized for cell stimulation. Considering fiber dimension, alignment, mechanical strength, electrical conductivity and biocompatibility, silk-CNT fibers containing a minute amount of CNT (0.05%) outperformed other fiber types. The modulation effect of these fibers was examined by their application in inducing polarization and activation of fibroblasts with cellular deficit. While the fibroblasts on both collagen-CNT and silk-CNT fibers synthesized a substantially higher level of collagen type III (COLIII) than cells on pure protein fibers to reduce the abnormally high COLI/COLIII ratio, electrical stimulation boosted the collagen productivity by 20 folds in cells on silk-CNT than on collagen-CNT due to silk-CNT's high electrical conductivity. The developed approach can be potentially utilized to remedy the dysfunctional fibroblasts for therapeutic treatment of diseases and health conditions associated with collagen disorder. (C) 2018 Elsevier Inc. All rights reserved.