Electron Microscopic Observations on the Effects of Penicillin on the Morphology of Chlamydia psittaci
Electron Microscopic Observations on the Effects of Penicillin on the Morphology of Chlamydia psittaci
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青霉素对鹦鹉热衣原体形态影响的电镜观察
DOI：
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发表时间：
1970
影响因子：
3.2
通讯作者：
G. P. Manire
中科院分区：
文献类型：
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作者：
A. Matsumoto;G. P. Manire
L细胞被脑膜炎生物以高倍数感染，并在含有200单位/ ml青霉素的培养基中培养。在感染后48小时内，取出细胞，制备薄片，用电子显微镜观察发育中的生物体的形态。青霉素对感染初级体形成发育网状体（RB）的初始重组没有影响，并且在感染后12小时内，处理过的和未处理过的培养物完全相同。然而，在那之后，青霉素处理过的生物体表现出显著的差异，二元裂变被阻止，大量的大的异常RB形式被看到，RB本身内外形成了大量的RB细胞质膜和包膜，大量空的或部分填充的小泡被从RB上夹走。36小时后，RB内形成未成熟的类核。在整个过程中，RB的外包膜和细胞质膜都被识别。当被感染的细胞被转移到无青霉素的培养基中时，异常RB表现出恢复形成正常RB的过程，包括发芽样过程和内部破碎或细分，如胞内形成。我们已经得出结论，青霉素抑制二元裂变，并阻止形成基本体包膜所必需的某些成分的合成。
L cells were infected at high multiplicity with meningopneumonitis organisms and incubated in medium containing 200 units per ml of penicillin. At intervals up to 48 hr after infection, cells were removed and thin sections were prepared for electron microscopic studies on the morphology of the developing organism. Penicillin had no effect on the initial reorganization of the infecting elementary body to form the developmental reticulate body (RB), and, up to 12 hr after infection, the treated and untreated cultures were identical. After that time, however, penicillin-treated organisms showed striking differences in that binary fission was prevented, large abnormal RB forms were seen in great numbers, masses of RB cytoplasmic membranes and envelopes were formed within and outside the RB itself, and large numbers of empty or partially filled small vesicles were pinched off the RB. After 36 hr immature nucleoids were formed within the RB. Throughout all of this period, both the outer cell envelope and the cytoplasmic membrane of these RB were recognized. When infected cells were transferred into penicillin-free medium, the abnormal RB showed recovery to form normal RB both by a budding-like process and by internal fragmentation or subdivision rather like endosporulation. We have concluded that penicillin inhibits binary fission and prevents the synthesis of certain components essential for the formation of the elementary body envelope.