An Electron Spin Resonance Study for Real-time Detection of Ascorbyl Free Radicals After Addition of Dimethyl Sulfoxide in Murine Hippocampus or Plasma During Kainic Acid-Induced Seizures
An Electron Spin Resonance Study for Real-time Detection of Ascorbyl Free Radicals After Addition of Dimethyl Sulfoxide in Murine Hippocampus or Plasma During Kainic Acid-Induced Seizures
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DOI：
10.1007/s11064-010-0148-9
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发表时间：
2010-07-01
影响因子：
4.4
通讯作者：
Yokoi, Isao
中科院分区：
文献类型：
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作者：
Matsumoto, Shigekiyo;Shingu, Chihiro;Yokoi, Isao
电子自旋共振(ESR)-沉默抗坏血酸溶液在加入摩尔过量的二甲亚砜（DMSO）后立即产生可检测的抗坏血酸自由基（AFR）的浓度依赖性信号。我们的目的是在添加DMSO （AFR/DMSO）后对AFR进行实时定量ESR分析，以评估全身给药海钠酸盐后小鼠新鲜海马或血浆中的抗坏血酸浓度。使用一种特殊的组织型石英细胞可以立即检测小鼠新鲜组织中的AFR/DMSO ESR光谱。kainic酸诱导小鼠癫痫发作时，新鲜海马或血浆中AFR/DMSO含量显著升高，在腹腔注射50mg /kg kainic酸90 min后达到最高水平。这表明海马体的氧化损伤是由于给药后大脑中大量抗坏血酸的积累造成的。在ESR光谱仪上测量的AFR/DMSO含量可用于新鲜组织中抗坏血酸含量的实时评估。该方法简便、性能好、成本低、可实时监测抗坏血酸，未来可应用于临床研究和治疗。
Electron spin resonance (ESR)-silent ascorbate solutions generate a detectable, likely concentration-dependent signal of ascorbyl free radicals (AFR) immediately upon addition of a molar excess of dimethyl sulfoxide (DMSO). We aimed to perform quantitative ESR analysis of AFR in real time after addition of DMSO (AFR/DMSO) to evaluate ascorbate concentrations in fresh hippocampus or plasma following systemic administration of kainate in mice. Use of a special tissue-type quartz cell allowed immediate detection of AFR/DMSO ESR spectra in fresh tissues from mice. AFR/DMSO content was increased significantly in fresh hippocampus or plasma obtained during kainate-induced seizures of mice, reaching maximum levels at 90 min after intraperitoneal administration of 50 mg/kg kainic acid. This suggests that oxidative injury of the hippocampus resulted from the accumulation of large amounts of ascorbic acid in the brain after kainic acid administration. AFR/DMSO content measured on an ESR spectrometer can be used for real-time evaluation of ascorbate content in fresh tissue. Due to the simplicity, good performance, low cost and real-time monitoring of ascorbate, this method may be applied to clinical research and treatment in the future.