Freezing as a method of sample preservation for the analysis of dissolved inorganic nutrients in seawater
Freezing as a method of sample preservation for the analysis of dissolved inorganic nutrients in seawater
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DOI：
10.1016/0304-4203(96)00004-7
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发表时间：
1996-08-01
期刊：
影响因子：
3
通讯作者：
Karl, DM
中科院分区：
文献类型：
--
作者：
Dore, JE;Houlihan, T;Karl, DM
在分析溶解的无机营养物之前，通常需要或必须储存收集的海水样品。因此，重要的是建立保存和储存技术，以确保样品的完整性，不会改变分析的精密度或准确度。我们已经进行了一系列的实验，在贫营养北太平洋基准站ALOHA收集的营养样品的存储，使用标准的自动分析和低水平的技术。我们的研究结果表明，对于贫营养的海洋沃茨，立即冷冻的未过滤的水样在一个干净的聚乙烯瓶是一个合适的保存方法。该程序简单，避免了潜在的污染样品操作和化学添加，并且充分保留了单个水样中硝酸盐+亚硝酸盐、可溶性反应性磷酸盐和可溶性反应性硅酸盐的浓度。
It is often desirable or necessary to store collected seawater samples prior to analysis for dissolved inorganic nutrients. It is therefore important to establish preservation and storage techniques that will ensure sample integrity and will not alter the precision or accuracy of analysis. We have performed a series of experiments on the storage of nutrient samples collected at the oligotrophic North Pacific benchmark Station ALOHA, using both standard autoanalyses and low-level techniques. Our results reveal that for oligotrophic oceanic waters, the immediate freezing of an unfiltered water sample in a clean polyethylene bottle is a suitable preservation method. This procedure is simple, it avoids potentially contaminating sample manipulations and chemical additions, and it adequately preserves the concentrations of nitrate + nitrite, soluble reactive phosphate, and soluble reactive silicate within a single water sample.