Genetics of receptors for bioactive polypeptides: expression of the human EGF receptor gene and internalization and processing of the receptor-bound EGF in human-mouse cell hybrids.
Genetics of receptors for bioactive polypeptides: expression of the human EGF receptor gene and internalization and processing of the receptor-bound EGF in human-mouse cell hybrids.
复制标题
生物活性多肽受体的遗传学：人 EGF 受体基因的表达以及人-小鼠细胞杂种中受体结合的 EGF 的内化和加工。
DOI：
10.1007/bf01538892
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Shimizu,N
中科院分区：
文献类型：
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作者：
Behzadian,MA;Shimizu,Y;Kondo,I;Shimizu,N
我们之前假设表皮生长因子（EGF）受体的结构基因位于人类7号染色体上（1，2）。在这项研究中，EGF受体和某些postreceptor功能进一步分析了一个独特的细胞杂交系，C2 B5，保留只有一个人的染色体的X;7易位除了一个几乎完整的小鼠亲本基因组。[125 I]EGF与C2 B5杂交细胞结合的动力学和Scatchard分析表明，它们携带单一类别的EGF受体，解离常数为4×10− 10 M。在杂交体中表达的受体被证明是免疫学上的人类本性。人表皮生长因子受体现在基本上嵌入小鼠质膜受到“下调”介导的配体表皮生长因子。细胞结合的EGF的分析表明，内化和加工发生在人-小鼠细胞杂交。EGF的降解似乎是通过溶酶体途径，因为它基本上被lysosomotropic剂延迟或抑制。
We previously postulated that the structural gene for epidermal growth factor (EGF) receptor is located on human chromosome 7 (1, 2). In this study, EGF receptor and certain postreceptor functions were further analyzed in a unique cell hybrid line, C2B5, that retains only one human chromosome of an X;7 translocation besides a nearly complete mouse parental genome. Kinetics and Scatchard analysis of [125I]EGF binding to the C2B5 hybrid cells indicated that they carry a single class of EGF receptors with a dissociation constant of 4×10−10M. The receptors expressed in the hybrids are proven to be immunologically of human nature. The human EGF receptors now embedded in essentially mouse plasma membrane are subject to “down regulation” mediated by the ligand EGF. Analysis of the cell-bound EGF indicated that internalization and processing take place in the human-mouse cell hybrids. The degradation of EGF appears to be through a lysosomal pathway since it was substantially delayed or inhibited by lysosomotropic agents.