Transcription of a bacteriophage lambda DNA site blocks growth of Escherichia coli.
Transcription of a bacteriophage lambda DNA site blocks growth of Escherichia coli.
复制标题
噬菌体 lambda DNA 位点的转录可阻断大肠杆菌的生长。
DOI：
10.1128/jb.172.2.1030-1034.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Guarneros,G
中科院分区：
文献类型：
--
作者：
Guzman,P;RiveraChavira,BE;Court,DL;Gottesman,ME;Guarneros,G
大肠杆菌中的RAP突变阻止了噬菌体lambda的生长。克服RAP抑制(BAR)的噬菌体突变已被定位到PL操纵子的基因座上。我们克隆并测序了其中两个基因座的三个突变：位于lambda连接位点(AttP)左臂的Baria和SSB(Ea10)基因中的Barii。这些突变代表了几乎相同的16个碱基对DNA片段中的单碱基对变化。每个突变都会破坏片段内的二联体对称性序列。在活性启动子下游携带bar+序列的质粒对RAP细菌是致命的，但对RAP+细菌不是。从lambda bar突变体中分离出的bar序列不是致命性的。我们合成了一个最小的lambda Baria+序列，5‘-TATATTGATATTTTATATCATT，并将其克隆到下游的可诱导启动子上。当转录时，这个序列足以杀死一个说唱菌株。
The rap mutation in Escherichia coli prevents the growth of bacteriophage lambda. Phage mutations that overcome rap inhibition (bar) have been mapped to loci in the pL operon. We cloned and sequenced three mutations in two of these loci: barIa to the left arm of the lambda attachment site (attP) and barII in the ssb (ea10) gene. The mutations represent single base-pair changes within nearly identical 16-base-pair DNA segments. Each mutation disrupts a sequence of dyad symmetry within the segment. Plasmids carrying a bar+ sequence downstream to an active promoter are lethal to rap, but not rap+, bacteria. The bar sequences isolated from the lambda bar mutants are not lethal. We synthesized a minimal lambda barIa+ sequence, 5'-TATATTGATATTTATATCATT, and cloned it downstream to an inducible promoter. When transcribed, this sequence is sufficient to kill a rap strain.