Platelet-rich plasma - Growth factor enhancement for bone grafts
Platelet-rich plasma - Growth factor enhancement for bone grafts
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DOI：
10.1016/s1079-2104(98)90029-4
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发表时间：
1998-06-01
影响因子：
--
通讯作者：
Georgeff, KR
中科院分区：
文献类型：
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作者：
Marx, RE;Carlson, ER;Georgeff, KR
富血小板血浆是血小板衍生生长因子和转化生长因子β的自体来源，可通过梯度密度离心分离和浓缩血小板获得。该技术产生了浓度为338%的人血小板，并在其中鉴定出血小板衍生生长因子和转化生长因子β。单克隆抗体对骨髓移植物松质细胞的评估表明，细胞能够通过携带细胞膜受体对生长因子产生反应。通过向移植物中添加富血小板血浆而获得的这些生长因子的额外量证明了放射线成熟率是不添加富血小板血浆移植物的1.62至2.16倍。通过组织形态学评估，添加富血小板血浆的移植物的骨密度（74.0% +/- 11%）也高于未添加富血小板血浆的移植物（55.1% +/- 8%;p = 0.005）。
Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. This technique produced a concentration of human platelets of 338% and identified platelet-derived growth factor and transforming growth factor beta within them. Monoclonal antibody assessment of cancellous cellular marrow grafts demonstrated cells that were capable of responding to the growth factors by bearing cell membrane receptors. The additional amounts of these growth factors obtained by adding platelet-rich plasma to grafts evidenced a radiographic maturation rate 1.62 to 2.16 times that of grafts without platelet-rich plasma. As assessed by histomorphometry, there was also a greater bone density in grafts in which platelet-rich plasma was added (74.0% +/- 11%)than in grafts in which platelet-rich plasma was not added (55.1% +/- 8%; p = 0.005).