Highly efficient biocatalytic cascade for the diversity-oriented synthesis of complex blood group Sd(a) antigens
Highly efficient biocatalytic cascade for the diversity-oriented synthesis of complex blood group Sd(a) antigens
复制标题
用于复杂血型 Sd(a) 抗原多样性合成的高效生物催化级联
DOI：
10.1039/d0gc02971h
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发表时间：
2020
期刊：
影响因子：
9.8
通讯作者：
Liu Chang-Cheng
中科院分区：
文献类型：
--
作者：
Zhong Kan;Ye Jinfeng;Zhu Xinhao;Cao Hongzhi;Liu Chang-Cheng
Sda抗原是一个分布广泛的组织血型抗原家族，具有Siaα2，3(GalNAcβ1，4)半乳糖三糖决定簇。尽管它们具有重要的生物学意义，但这些复杂碳水化合物抗原的合成是具有挑战性的。在这里，我们报道了一种利用6个多酶串联反应系统高效、绿色地合成具有不同基础结构和唾液酸形式的各种SDA抗原的方法。多酶串联的方法结合了糖核苷酸供体生成酶和糖基转移酶，为从简单的单糖构建块快速组装复杂的多糖提供了一种有效和方便的方法。
Sda antigens are a family of widely distributed histo-blood group antigens sharing the Siaα2,3(GalNAcβ1,4)Gal trisaccharide determinant. Despite their significant biological importance, the synthesis of these complex carbohydrate antigens is challenging. Herein, we report a highly efficient and green synthesis of diverse Sda antigens with different underlying structures and sialic acid forms utilizing 6 multienzyme cascade reaction systems. The multienzyme cascade approach combines sugar nucleotide donor generation enzymes and glycosyltransferases, providing an efficient and convenient approach for the rapid assembly of complex glycans from simple monosaccharide building blocks.