SPLITTING OF HEPATOCYTE GAP JUNCTIONS AND ZONULAE OCCLUDENTES WITH HYPERTONIC DISACCHARIDES
SPLITTING OF HEPATOCYTE GAP JUNCTIONS AND ZONULAE OCCLUDENTES WITH HYPERTONIC DISACCHARIDES
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DOI：
10.1083/jcb.61.3.575
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发表时间：
1974-01-01
影响因子：
7.8
通讯作者：
GILULA, NB
中科院分区：
文献类型：
--
作者：
GOODENOUGH, DA;GILULA, NB
通过门静脉用高渗浓度 (0. 5 M) 的二糖蔗糖、乳糖、麦芽糖和纤维二糖对小鼠肝脏进行原位灌注。这种处理导致肝细胞质壁分离以及间隙连接和闭塞小带的分裂。连接点对称地分开，在两个分离的单元上各留下一个半连接点。使用冷冻断裂技术跟踪连接分裂过程，因为一旦发生分裂，连接膜与薄切片中的非连接膜就无法区分。还使用冷冻蚀刻技术研究了分裂连接，从而可以观察通常隔离在 2 nm 间隙内的间隙连接细胞外表面。”单糖山梨醇和甘露醇在研究时间（2 分钟）内不会分裂连接，但在灌注混合物中用丙酸根取代氯离子确实导致连接分裂。还描述了围绕冷冻断裂间隙连接的形态不同的颗粒的包膜。
Mouse livers were perfused in situ through the portal vein with the disaccharides sucrose, lactose, maltose, and cellobiose in hypertonic concentrations (0. 5 M). This treatment resulted in plasmolysis of the hepatocytes and splitting of the gap junctions and zonulae occludentes. The junctions split symmetrically, leaving a half-junction on each of the two separated cells. The process of junction splitting is followed using the freeze-fracture technique, since the junctional membranes are indistinguishable from the nonjunctional membranes in-thin sections once the splitting occurs. The split junctions are also studied using the freeze-etch technique, allowing a view of the gap junction extracellular surface normally sequestered within the 2-nm" gap." The monosaccharides sorbitol and mannitol did not split the junctions during the times studied (2 min), but substitution of the chloride ion with propionate in the perfusion mixture did result in junction splitting. An envelope of morphologically distinct particles surrounding freeze-fractured gap junctions is also described.