Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
复制标题
章鱼碱型冠瘿瘤中几种质粒 DNA 编码转录物的大小和图谱位置。
DOI：
10.1073/pnas.79.1.76
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发表时间：
1982
影响因子：
11.1
通讯作者：
Nester,EW
中科院分区：
文献类型：
--
作者：
Gelvin,SB;Thomashow,MF;McPherson,JC;Gordon,MP;Nester,EW
用斑点杂交法测定了几种章鱼素型烟草冠瘤的质粒DNA编码的RNA分子的大小和相对图谱位置。在产生章鱼碱的肿瘤(系A6S/2、E9和15955/01)中，至少检测到6个离散的多腺苷化RNA(大小为0.73-1.75kb)。在一个不能检测到章鱼的肿瘤(15955/1行)中，一个核糖核酸物种缺失，另一个物种丰度降低。有证据表明，转录本完全由质粒DNA编码，而不是来自周围的植物DNA序列。
The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors were determined by blot hybridization. At least six discrete polyadenylylated RNAs (sizes 0.73-1.75 kilobases) were detected in octopine-producing tumors (lines A6S/2, E9, and 15955/01). In one tumor (line 15955/1) in which octopine could not be detected, one of the RNA species was missing and another was diminished in abundance. Evidence is presented suggesting that the transcripts are encoded entirely by the plasmid DNA and do not originate from the surrounding plant DNA sequences.