Chip calorimetry and its use for biochemical and cell biological investigations
Chip calorimetry and its use for biochemical and cell biological investigations
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DOI：
10.1016/j.cep.2007.02.014
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发表时间：
2008-06-01
影响因子：
4.3
通讯作者：
Wolf, Gert
中科院分区：
文献类型：
--
作者：
Lerchner, Johannes;Maskow, Thomas;Wolf, Gert
本文分析了芯片量热法在生物化学和细胞生物学研究中的潜力。从芯片量热计热功率探测器的热物理性质可以推断，其应用主要是指极小样品的快速反应。相比之下，使用芯片量热计研究缓慢的过程，如生物化学或微生物反应受到限制。然而，仔细优化芯片热量计的信号分辨率和样品体积，使酶催化反应的调查和微生物生长热的测量，随后证明了一些典型的例子从文献中。(C)2007 Elsevier B.V.保留所有权利。
The presented paper analyzes the potential of chip calorimetry for biochemical and cell biological investigations. From the thereto-physical properties of the heat power detectors of a chip calorimeter can be deduced that relevant applications mainly refer to fast reactions in extremely small samples. In contrast, the use of chip calorimeters for studying slow processes such as biochemical or microbial reactions is restricted. However, careful optimization of chip calorimeters concerning signal resolution and sample volume enables the investigation of enzymatically catalyzed reactions and the measurement of microbial growth heat as subsequently demonstrated with some typical examples from the literature. (C) 2007 Elsevier B.V. All rights reserved.