Diagnostic, Prognostic, and Therapeutic Value of Droplet Digital PCR (ddPCR) in COVID-19 Patients: A Systematic Review.
Diagnostic, Prognostic, and Therapeutic Value of Droplet Digital PCR (ddPCR) in COVID-19 Patients: A Systematic Review.
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DOI：
10.3390/jcm10235712
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发表时间：
2021-12-06
影响因子：
3.9
通讯作者：
Nikas IP
中科院分区：
文献类型：
--
作者：
Ishak A;AlRawashdeh MM;Esagian SM;Nikas IP
准确检测引起COVID-19全球大流行的病原体SARS-CoV-2对疾病监测和控制至关重要。定量反转录PCR （RT-qPCR）被世界卫生组织和美国疾病控制与预防中心认为是诊断SARS-CoV-2的参考标准检测。然而，它的局限性促使人们采用更准确的检测方法来检测SARS-CoV-2，量化其水平并评估预后。本文旨在系统回顾文献，评估液滴数字PCR （ddPCR）的诊断性能，并评估其在COVID-19患者预后和管理中的潜在作用。检索PubMed和Scopus数据库以确定2021年7月13日之前发表的相关文章。2021年10月21日又进行了一次PubMed搜索。从39项符合条件的研究中提取数据，并使用来自2825名患者和145名对照的3651份样本进行定性分析。大多数研究报道，在检测和定量SARS-CoV-2水平方面，ddPCR比RT-qPCR更准确，特别是在病毒载量低的患者中。ddPCR还被发现在定量住院患者的SARS-CoV-2 RNAemia水平、监测其病程和预测其对治疗的反应方面非常有效。这些发现表明，ddPCR可以作为一种补充或替代SARS-CoV-2工具，具有新兴的诊断、预后和治疗价值，特别是在医院环境中。仍需要进一步的研究来规范其实验室方案，并准确评估其在监测COVID-19治疗反应和识别SARS-CoV-2新变体方面的作用。
Accurate detection of SARS-CoV-2, the pathogen causing the global pandemic of COVID-19, is essential for disease surveillance and control. Quantitative reverse transcription PCR (RT-qPCR) is considered the reference standard test for the diagnosis of SARS-CoV-2 by the World Health Organization and Centers for Disease Control and Prevention. However, its limitations are a prompt for a more accurate assay to detect SARS-CoV-2, quantify its levels, and assess the prognosis. This article aimed to systematically review the literature and assess the diagnostic performance of droplet digital PCR (ddPCR), also to evaluate its potential role in prognosis and management of COVID-19 patients. PubMed and Scopus databases were searched to identify relevant articles published until 13 July 2021. An additional PubMed search was performed on 21 October 2021. Data from the 39 eligible studies were extracted and an overall 3651 samples from 2825 patients and 145 controls were used for our qualitative analysis. Most studies reported ddPCR was more accurate than RT-qPCR in detecting and quantifying SARS-CoV-2 levels, especially in patients with low viral loads. ddPCR was also found highly effective in quantifying SARS-CoV-2 RNAemia levels in hospitalized patients, monitoring their disease course, and predicting their response to therapy. These findings suggest ddPCR could serve as a complement or alternative SARS-CoV-2 tool with emerging diagnostic, prognostic, and therapeutic value, especially in hospital settings. Additional research is still needed to standardize its laboratory protocols, also to accurately assess its role in monitoring COVID-19 therapy response and in identifying SARS-CoV-2 emerging variants.