In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material.
In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material.
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石墨烯增强纳米羟基磷灰石聚酰胺66三元生物复合材料作为骨科植入材料的体外和体内生物相容性和成骨作用
DOI：
10.2147/ijn.s105794
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发表时间：
2016
影响因子：
8
通讯作者：
Jiang D
中科院分区：
文献类型：
--
作者：
Zhang S;Yang Q;Zhao W;Qiao B;Cui H;Fan J;Li H;Tu X;Jiang D
石墨烯及其衍生物由于具有比表面积大、力学性能好等优良特性，在骨组织工程中的应用越来越受到人们的关注。在这项研究中，石墨烯增强纳米羟基磷灰石/聚酰胺66（nHA/PA 66）骨螺钉的制备。扫描电镜观察和X射线衍射数据表明，石墨烯和nHA在PA 66基体中均具有良好的分散性。复合材料的拉伸强度和弹性模量分别提高了49.14%和21.2%。小鼠骨髓间充质干细胞系C3 H10 T1/2在石墨烯增强的nHA/PA 66复合材料中表现出比nHA/PA 66复合材料更好的粘附和增殖。细胞发育出更多的伪足，具有更大的细胞密度和更可区分的细胞骨架结构。通过荧光染色和细胞活力测定证实了这些结果。C3 H10 T1/2细胞在成骨分化培养基中培养7天和14天后，与石墨烯增强的nHA/PA 66共培养的细胞中骨分化相关基因表达、碱性磷酸酶和骨钙素显著增加。该结果证明了该复合材料的骨诱导特性，茜素红染色结果进一步支持了这一发现。此外，将石墨烯增强的nHA/PA 66接骨螺钉植入犬股骨髁，术后组织学显示肝、脾、肾、脑或其他主要器官无明显损伤。种植体周围骨组织生长良好，与种植体直接连接。软组织未见明显炎症反应，证明螺钉具有良好的生物相容性。这些观察结果表明，石墨烯增强的nHA/PA 66复合材料在骨组织工程中具有很大的应用潜力。
Graphene and its derivatives have been receiving increasing attention regarding their application in bone tissue engineering because of their excellent characteristics, such as a vast specific surface area and excellent mechanical properties. In this study, graphene-reinforced nanohydroxyapatite/polyamide66 (nHA/PA66) bone screws were prepared. The results of scanning electron microscopy observation and X-ray diffraction data showed that both graphene and nHA had good dispersion in the PA66 matrix. In addition, the tensile strength and elastic modulus of the composites were significantly improved by 49.14% and 21.2%, respectively. The murine bone marrow mesenchymal stem cell line C3H10T1/2 exhibited better adhesion and proliferation in graphene reinforced nHA/PA66 composite material compared to the nHA/PA66 composites. The cells developed more pseudopods, with greater cell density and a more distinguishable cytoskeletal structure. These results were confirmed by fluorescent staining and cell viability assays. After C3H10T1/2 cells were cultured in osteogenic differentiation medium for 7 and 14 days, the bone differentiation-related gene expression, alkaline phosphatase, and osteocalcin were significantly increased in the cells cocultured with graphene reinforced nHA/PA66. This result demonstrated the bone-inducing characteristics of this composite material, a finding that was further supported by alizarin red staining results. In addition, graphene reinforced nHA/PA66 bone screws were implanted in canine femoral condyles, and postoperative histology revealed no obvious damage to the liver, spleen, kidneys, brain, or other major organs. The bone tissue around the implant grew well and was directly connected to the implant. The soft tissues showed no obvious inflammatory reaction, which demonstrated the good biocompatibility of the screws. These observations indicate that graphene-reinforced nHA/PA66 composites have great potential for application in bone tissue engineering.