In vivo cloning and characterization of a new growth suppressor protein TOE1 as a direct target gene of EGR1
In vivo cloning and characterization of a new growth suppressor protein TOE1 as a direct target gene of EGR1
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DOI：
10.1074/jbc.m210502200
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发表时间：
2003-04-18
影响因子：
4.8
通讯作者：
Adamson, ED
中科院分区：
文献类型：
--
作者：
de Belle, I;Wu, JX;Adamson, ED
Egr1是一种即刻早期转录因子，能对多种刺激做出反应，影响基因转录，从而实现其生物学效应。Egr1表达的一个重要作用是降低几种肿瘤细胞的生长和致瘤潜能。为了确定重要的Egr1靶基因，我们采用了一种方法，包括甲醛诱导的蛋白质-DNA交联、染色质免疫沉淀和多重PCR。利用这种方法，我们报道了一个新的Egr1靶基因的克隆，该基因至少能够部分解释Egr1的生长抑制活性。我们将这个新蛋白命名为TOE1，作为Egr1的靶标。
Egr1, an immediate early transcription factor, responds to diverse stimuli and affects gene transcription to accomplish its biological effects. One important effect of Egr1 expression is to decrease the growth and tumorigenic potential of several tumor cell types. To identify important Egr1 target genes, we have adapted a methodology involving formaldehyde-induced protein-DNA cross-linking, chromatin immunoprecipitation, and multiplex PCR. Using this approach, we report the cloning of a new Egr1 target gene that is able to account, at least in part, for the growth inhibitory activity of Egr1. We have named this new protein TOE1 for target of Egr1.