Y2SK2 and SK3 type dehydrins from Agapanthus praecox can improve plant stress tolerance and act as multifunctional protectants
Y2SK2 and SK3 type dehydrins from Agapanthus praecox can improve plant stress tolerance and act as multifunctional protectants
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百子莲中的 Y2SK2 和 SK3 型脱水素可提高植物的抗逆性并作为多功能保护剂
DOI：
10.1016/j.plantsci.2019.03.012
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Zhang Di
中科院分区：
文献类型：
--
作者：
Yang Zhou;Sheng Jiangyuan;Lv Ke;Ren Li;Zhang Di
百子莲中的两种抗氧化蛋白ApY 2SK 2和ApSK 3在复合胁迫下表现出重要的保护作用。ApY 2SK 2和ApSK 3均含有一个内含子，分别由981 bp和1057 bp的全长cDNA组成，编码186和215个氨基酸。ApY 2SK 2和ApSK 3主要定位于细胞质和细胞膜，ApY 2SK 2甚至可以定位于细胞核，体外试验表明，ApY 2SK 2和ApSK 3在冻融过程中可以有效地保护酶活性，ApY 2SK 2在干燥处理中也表现出这种功能。此外，ApY 2SK 2和ApSK 3还能显著抑制羟自由基的产生。这两种蛋白与金属离子的结合亲和力大小为Co2+> Ni 2 +> Cu 2 +> Fe 3+，ApSK 3的结合亲和力高于ApY 2SK 2。因此，ApY 2SK 2对酶活性具有较好的保护作用，ApSK 3具有较强的金属离子结合功能和对ROS代谢的影响。此外，植物超低温保存评价试验表明，ApY 2SK 2和ApSK 3转化可使幼苗存活率分别从23%提高到47%和55%，在植物玻璃化液中加入重组ApY 2SK 2和ApSK 3可提高野生型A的存活率。对海兰幼苗生长的抑制率分别从24%提高到50%和46%。这些结果表明，ApY 2SK 2和ApSK 3能有效提高细胞的应激耐受性，具有很大的体内或体外应用潜力。
Two dehydrins fromAgapanthus praecox(ApY2SK2andApSK3) show important protective effects under complex stresses. Both ApY2SK2and ApSK3contain one intron and consist of a full-length cDNA of 981 bp and 1057 bp encoding 186 and 215 amino acids, respectively.ApY2SK2andApSK3transgenicArabidopsis thalianashow reduced plasma membrane damage and ROS levels and higher antioxidant activity and photosynthesis capability under salt, osmotic, cold and drought stresses compared with the wild-type. ApY2SK2and ApSK3are mainly located in the cytoplasm and cell membrane, and ApY2SK2can even localize in the nucleus.In vitrotests indicate that ApY2SK2and ApSK3can effectively protect enzyme activity during the freeze-thaw process, and ApY2SK2also exhibits this function during desiccation treatment. Furthermore, ApY2SK2and ApSK3can significantly inhibit hydroxyl radical generation. These two dehydrins can bind metal ions with a binding affinity of Co2+> Ni2+> Cu2+> Fe3+; the binding affinity of ApSK3is higher than that of ApY2SK2. Thus, ApY2SK2has a better protective effect on enzyme activity, and ApSK3has stronger metal ion binding function and effect on ROS metabolism. Moreover, plant cryopreservation evaluation tests indicate thatApY2SK2andApSK3transformation can enhance the seedling survival ratio from 23% to 47% and 55%, respectively; the addition of recombinant ApY2SK2and ApSK3to plant vitrification solution may increase the survival ratio of wild-typeA. thalianaseedlings from 24% to 50% and 46%, respectively. These findings suggest that ApY2SK2and ApSK3can effectively improve cell stress tolerance and have great potential forin vivoorin vitroapplications.