Genotype profile of human polyomavirus JC excreted in urine of immunocompetent individuals
Genotype profile of human polyomavirus JC excreted in urine of immunocompetent individuals
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DOI：
10.1128/jcm.34.1.159-164.1996
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发表时间：
1996-01-01
影响因子：
9.4
通讯作者：
Stoner, GL
中科院分区：
文献类型：
--
作者：
Agostini, HT;Ryschkewitsch, CF;Stoner, GL
人类多瘤病毒JC（JCV）可导致约5%的AIDS患者发生中枢性脱髓鞘疾病进行性多灶性白质脑病。为了表征美国对照人群中JCV的类型特征，通过PCR随后直接循环测序检查了54名女性（10至79岁;平均年龄43.4岁）和51名男性（18至94岁;平均年龄47.9岁）尿液中不同基因型JCV的排泄。研究对象为89名普通门诊患者和16名健康志愿者。JC病毒尿的总发病率为43/105（40.9%）例受试者，30岁以上受试者的发病率显著增加。发现两名男子同时排出两种不同类型的JCV，表明双重感染。在迄今为止鉴定的三种不同基因型JCV中，1型菌株（欧洲）在该队列中最常见（占总菌株的64%），其次是2型（东亚）（18%）。未检测到3型（东非）菌株。在7名个体（16%）中以1/3型重组体（也称为4型）的形式发现了存在JCV 3型的间接证据。此外，在一名59岁的非洲裔美国人中发现了一个与1型、2型和3型不同的JCV，可能代表了一种遗传学上较老的类型（5型）。描述JCV类型之间的序列变异对于设计用于临床样品的灵敏PCR的引物是必不可少的。
The human polyomavirus JC (JCV) causes the central demyelinating disease progressive multifocal leukoencephalopathy in about 5% of AIDS patients. To characterize the type profile of JCV in a control population in the United States, 54 females (10 to 79 years of age; average age, 43.4 years) and 51 males (18 to 94 years of age; average age, 47.9 years) were examined for the excretion of different genotypes of JCV in their urine by PCR followed by direct cycle sequencing. The group consisted of 89 patients of a general medical clinic in addition to 16 healthy volunteers. The overall incidence of JC viruria was 43 of 105 (40.9%) subjects, with a marked increase for those subjects above the age of 30 years. Two men were found to excrete two different types of JCV at the same time, indicating double infections. Of the three different genotypes of JCV identified to date, type 1 strains (European) were the most common in this cohort (64% of total strains) followed by type 2 (East Asian) (18%). No type 3 (East African) strains were detected. Indirect evidence for the existence of JCV type 3 was found in seven individuals (16%) in the form of a type 1/3 recombinant (also called type 4). In addition, a single example of JCV which differs from types 1, 2, and 3 and may represent a phylogenetically older type (type 5) was found in a 59-year-old African-American. Delineation of sequence variations between JCV types is essential for the design of primers for sensitive PCR with clinical samples.