Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres
Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres
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DOI：
10.1002/jat.1486
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发表时间：
2010-04-01
影响因子：
3.3
通讯作者：
Ghandehari, Hamidreza
中科院分区：
文献类型：
--
作者：
Arnida;Malugin, Alexander;Ghandehari, Hamidreza
使用一系列的金纳米粒子的尺寸递增，但不同的几何形状（球形与杆），我们已经评估了形状，大小，表面性质和浓度对细胞摄取，蛋白质的吸附和毒性在人前列腺癌细胞系（PC-3）的影响。在30-90 nm直径的范围内研究，球形粒子的SO纳米直径没有聚乙二醇（PEG）具有最高的吸收。PEG的表面附着降低了细胞摄取。聚乙二醇化的金纳米棒与它们的球形对应物相比具有净正电荷，并且颗粒几何形状影响细胞摄取。在没有血清蛋白的情况下，增加了对平面球形GNP的摄取。这些研究为定制金纳米粒子用于靶向肿瘤治疗应用铺平了道路。版权所有（C）2009约翰威利父子有限公司
Using a series of gold nanoparticles with incremental increase in dimensions but varying geometries (spherical vs rods) we have evaluated the influence of shape, size, surface properties and concentration on cellular uptake, adsorption of proteins and toxicity in a human prostate cancer cell line (PC-3). In the range of 30-90 nm diameter studied, spherical particles of SO nm in diameter without polyethylene glycol (PEG) had the highest uptake. Surface attachment of PEG reduced cellular uptake. PEGylated gold nanorods had a net positive charge compared with their spherical counterparts and particle geometry influenced cellular uptake. In the absence of serum proteins the uptake of plain spherical GNPs increased. These studies pave the way for the tailoring of gold nanoparticles for targeted tumor therapy applications. Copyright (C) 2009 John Wiley & Sons, Ltd.