Modulation of piezoelectric properties in electrospun PLLA nanofibers for application-specific self-powered stem cell culture platforms
Modulation of piezoelectric properties in electrospun PLLA nanofibers for application-specific self-powered stem cell culture platforms
复制标题
电纺PLLA纳米纤维压电性能的调控及其在自供电干细胞培养平台中的应用
DOI：
10.1016/j.nanoen.2021.106444
复制
发表时间：
2021-08-26
期刊：
影响因子：
17.6
通讯作者：
Nam, Jin
中科院分区：
文献类型：
--
作者：
Tai, Youyi;Yang, Steve;Nam, Jin
在再生医学和组织工程应用中，越来越多的努力利用压电材料作为自供电平台来电刺激细胞/组织。聚L-乳酸（PLLA）具有良好的生物降解性，特别是通过静电纺丝制备的纳米纤维状材料，在生物领域具有巨大的应用潜力。然而，其实现和压电转换的机制还没有得到很好的理解。在这项研究中，采用实验设计的方法，系统地剖析尺寸控制和热处理对静电纺聚乳酸纳米纤维的压电性能的影响。具体地说，我们发现，纤维直径和热处理相关的相含量之间的变化静电纺丝诱导的非晶和结晶α/α '相是负责在横向和纵向方向上的压电性能。PLLA纳米纤维中压电特性的这种调制在以表型特异性方式确定干细胞的分化效率中是至关重要的，其中神经发生和骨发生分别通过正交和剪切压电性来增强。总的来说，我们的研究结果突出了电纺PLLA纳米纤维的潜力，其具有精确控制的压电性能，通过系统的方法用于自供电的干细胞工程平台，特定于靶组织。
There is an increasing effort to utilize piezoelectric materials as a self-powered platform to electrically stimulate cells/tissues in regenerative medicine and tissue engineering applications. Poly(L-lactic acid) (PLLA) holds great potential for biological applications due to its biodegradability, especially in a nanofibrous form prepared by electrospinning. However, the mechanism underlying its realization and transformation of piezoelectricity is not well understood. In this study, a design-of-experiment approach was employed to systematically dissect the effects of dimensional control and heat treatment on the piezoelectric performance of electrospun PLLA nanofibers. Specifically, we revealed that the fiber diameter- and heat treatment-dependent phase content change between electrospinning-induced amorphous and crystalline alpha/alpha' phases was responsible for the piezoelectric performance in the transverse and longitudinal directions. Such modulation of piezoelectric properties in PLLA nanofibers was critical in determining the differentiation efficiency of stem cells in a phenotype-specific manner, where neurogenesis and osteogenesis were enhanced by orthogonal and shear piezoelectricity, respectively. Overall, our findings highlight the potential of electrospun PLLA nanofibers with precisely controlled piezoelectric properties through a systematic approach for self-powered stem cell engineering platforms, specific to target tissues.