Translation initiation factor IF1 of Bacillus stearothermophilus and Thermus thermophilus substitute for Escherichia coli IF1 in vivo and in vitro without a direct IF1-IF2 interaction.
Translation initiation factor IF1 of Bacillus stearothermophilus and Thermus thermophilus substitute for Escherichia coli IF1 in vivo and in vitro without a direct IF1-IF2 interaction.
复制标题
嗜热脂肪芽孢杆菌和嗜热栖热菌的翻译起始因子 IF1 在体内和体外替代大肠杆菌 IF1，而没有直接的 IF1-IF2 相互作用。
DOI：
10.1111/j.1365-2958.2008.06466.x
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发表时间：
2008
影响因子：
3.6
通讯作者：
Pon,CynthiaL
中科院分区：
文献类型：
--
作者：
Kapralou,Stavroula;Fabbretti,Attilio;Garulli,Chiara;Spurio,Roberto;Gualerzi,ClaudioO;Dahlberg,AlbertE;Pon,CynthiaL
Bacterial translation initiation factor IF1 is homologous to archaeal aIF1A and eukaryal eIF1A, which form a complex with their homologous IF2‐like factors (aIF5B and eIF5B respectively) during initiation of protein synthesis. A similar IF1–IF2 interaction is assumed to occur in all bacteria and supported by cross‐linking data and stabilization of the 30S–IF2 interaction by IF1. Here we compareEscherichia coliIF1 with thermophilic factors fromBacillus stearothermophilusandThermus thermophilus. All three IF1s are structurally similar and functionally interchangeablein vivoandin vitro. However, the thermophilic factors do not stimulate ribosomal binding of IF2ΔN, regardless of 30S subunits and IF2 origin. We conclude that an IF1–IF2 interaction is not universally conserved and is not essential for cell survival.
