PARALLEL INDUCTION BY GLUCOSE OF ADHERENCE AND A POLYSACCHARIDE ANTIGEN SPECIFIC FOR PLASTIC-ADHERENT STAPHYLOCOCCUS-EPIDERMIDIS - EVIDENCE FOR FUNCTIONAL RELATION TO INTERCELLULAR-ADHESION
PARALLEL INDUCTION BY GLUCOSE OF ADHERENCE AND A POLYSACCHARIDE ANTIGEN SPECIFIC FOR PLASTIC-ADHERENT STAPHYLOCOCCUS-EPIDERMIDIS - EVIDENCE FOR FUNCTIONAL RELATION TO INTERCELLULAR-ADHESION
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DOI：
10.1128/iai.60.5.2048-2057.1992
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发表时间：
1992-05-01
影响因子：
3.1
通讯作者：
LAUFS, R
中科院分区：
文献类型：
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作者：
MACK, D;SIEMSSEN, N;LAUFS, R
表皮葡萄球菌的初始附着和积累的聚合物表面上的多层细胞簇嵌入在无定形粘液，这一起导致塑料粘附表型检测粘附试验在这项研究中使用的，已被提出是这些细菌的主要毒力因子。一种对塑料粘附性链球菌特异的抗原。用抗强塑料粘附性表皮葡萄球菌的吸附抗血清，通过间接免疫荧光试验检测表皮葡萄球菌。epidermidis 1457.建立了凝血试验，其允许在不同生理生长条件下定量细菌提取物中的抗原。抗原和塑料粘附的表达显著依赖于生长培养基中葡萄糖的存在。与塑料粘附增加平行，与在缺乏葡萄糖的TSB中生长的细胞提取物相比，在胰蛋白胨大豆肉汤（TSB）中生长的细胞的细菌提取物中检测到抗原量增加32至64倍。在葡萄糖刺激塑料粘附的S.在缺乏葡萄糖的TSB中生长的表皮葡萄球菌菌株。抗原最可能由多糖组成，因为其免疫反应性被高碘酸氧化完全消除，但对蛋白酶消化有抗性。与非贴壁S.在TSB中生长的表皮葡萄球菌菌株位于超过50个细胞的大细胞簇中，其在细胞制剂的高碘酸盐氧化后完全崩解。高碘酸盐原位氧化粘附的细菌膜导致从塑料表面释放粘附的细胞。这些结果有力地表明抗原与S.表皮细胞粘附于聚合物表面，最可能是通过介导细胞间粘附，导致在多层细胞簇中积聚。
The initial attachment and the accumulation of Staphylococcus epidermidis on polymer surfaces in multilayered cell clusters embedded in amorphous slime, which together lead to the plastic-adherent phenotype detected by the adherence assay used in this study, have been proposed to be major virulence factors of these bacteria. An antigen specific for plastic-adherent S. epidermidis strains was detected by an indirect immunofluorescence test using absorbed antiserum raised against the strongly plastic-adherent S. epidermidis 1457. A coagglutination assay was established, which allowed the quantitation of the antigen in bacterial extracts under different physiologic growth conditions. Expression of the antigen and of plastic adherence depended significantly on the presence of glucose in the growth medium. Parallel to increased plastic adherence, a 32- to 64-fold increase in the amount of the antigen was detected in bacterial extracts of cells grown in tryptone soya broth (TSB) compared with that in extracts of cells grown in TSB lacking glucose. A parallel time-dependent increase of plastic adherence and expression of the antigen was observed after stimulation by glucose of stationary-phase cultures of plastic-adherent S. epidermidis strains grown in TSB lacking glucose. The antigen consisted most probably of polysaccharide, because its immunologic reactivity was completely abolished by periodate oxidation but was resistant to protease digestion. A significant proportion of cells of plastic-adherent as compared with nonadherent S. epidermidis strains grown in TSB were located in large cell clusters exceeding 50 cells, which completely disintegrated after periodate oxidation of the cell preparations. Periodate oxidation of adherent bacterial films in situ led to release of the adherent cells from the plastic surface. These results strongly indicate a functional relation of the antigen to adherence of S. epidermidis to polymer surfaces, most probably by mediating intercellular adhesion of cells leading to accumulation in multilayered cell clusters.