Sero- and molecular typing of Duffy blood group in Southeast Asians and Oceanians.
Sero- and molecular typing of Duffy blood group in Southeast Asians and Oceanians.
复制标题
东南亚和大洋洲人达菲血型的血清和分子分型。
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
T. Ishida
中科院分区：
文献类型：
--
作者：
Y. Shimizu;H. Ao;A. Soemantri;D. Tiwawech;W. Settheetham;O. W. Kayame;M. Kimura;T. Nishioka;T. Ishida
Duffy血型在疟疾流行的泰国和印度尼西亚人群中进行了研究：苗族（n = 103），Akha（n = 218），Lisu（n = 44），Bugis（n = 95），Toraja（n = 77），Dani（n = 44），Mee（n = 80）和Irianese（n = 81）。表型研究采用顺序间接Coombs试验，基因型测定采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）试验。DNA分析用于确定达菲血型系统。根据表型推断的Fya等位基因频率（0.958-1.0）与东南亚和大洋洲的其他群体相似。该研究揭示了Fya-抗原的存在，显示出对抗血清的弱反应性，以及PCR-RFLP研究显示的基因型与表型预测的基因型之间的差异。PCR-RFLP研究还表明Fy（a- B-）表型存在替代遗传基础，与非洲型不同。
Duffy blood group was studied among malaria-endemic Thai and Indonesian populations: Hmong (n = 103), Akha (n = 218), Lisu (n = 44), Bugis (n = 95), Toraja (n = 77), Dani (n = 44), Mee (n = 80) and Irianese (n = 81). Phenotypes were studied by the ordinal indirect Coombs' test and genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test. DNA analysis was used to type the Duffy blood group system. Deduced allele frequencies of Fya (0.958-1.0) based on the phenotypes were similar to those in other populations in Southeast Asia and Oceania. The study revealed the presence of Fya-antigens showing weak reactivity to antisera as well as a discrepancy between the genotype shown by the PCR-RFLP study and that predicted by the phenotype. The PCR-RFLP study also suggested the presence of an alternative genetic basis for the Fy(a- b-) phenotype, which differs from the African type.
影响因子：
56.9
作者：
HORUK, R;CHITNIS, CE;MILLER, LH
通讯作者：
MILLER, LH