Gene Expression of Survivin and Its Spliced Isoforms Associated With Proliferation and Aggressive Phenotypes of Prostate Cancer
Gene Expression of Survivin and Its Spliced Isoforms Associated With Proliferation and Aggressive Phenotypes of Prostate Cancer
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DOI：
10.1016/j.urology.2007.12.064
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发表时间：
2008-12-01
期刊：
影响因子：
2.1
通讯作者：
Suzuki, Kazuhiro
中科院分区：
文献类型：
--
作者：
Koike, Hidekazu;Sekine, Yoshitaka;Suzuki, Kazuhiro
目的探讨Survivin基因表达对前列腺癌（PCa）细胞增殖的影响及Survivin及其剪接体基因表达水平与PCa病理分级的关系。我们使用小干扰RNA敲低存活素的基因表达，并使用MTS测定评估细胞增殖。接下来，我们定量前列腺活检样品（PCa，n = 37;良性前列腺）中存活素及其同种型的基因表达水平。结果在前列腺癌细胞中，Survivin、Survivin-2 α和Survivin-2B的表达高于Survivin-Delta Ex 3。siRNA转染后Survivin基因表达的降低伴随着PCa细胞增殖的抑制（LNCaP和PC-3细胞中分别降低了31%和25%，P
OBJECTIVES To assess the effect Of survivin gene expression on the proliferation of prostate cancer (PCa) cells and Study the association Of suvivin and its spliced isoforms gene expression levels with the pathologic grade of PCa.METHODS Gene expression Of survivin and its spliced isoforms in the LNCaP and PC-3 PCa cell lines was determined using reverse transcriptase-polymerase chain reaction. We knocked down the gene expression of survivin using small interfering RNA and assessed the cell proliferation using the MTS assay. Next, we quantified the gene expression levels Of survivin and its isoforms in prostate biopsy samples (PCa, n = 37; benign prostatic. hyperplasia, n = 13; PCa after androgen deprivation therapy, n = 12) Using the quantitative real-time polymerase chain reaction method.RESULTS In PCa cells, survivin and survivin-2 alpha and survivin-2B were expressed more than survivin-Delta Ex3. The decrease in survivin gene expression by transfection of siRNA was accompanied by the inhibition of cell proliferation of PCa cells (31% and 25% decreased in LNCaP and PC-3 cells, P