A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function
A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function
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DOI：
10.1128/mcb.17.4.2136
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发表时间：
1997-04-01
影响因子：
5.3
通讯作者：
Campbell, JL
中科院分区：
文献类型：
--
作者：
Budd, ME;Campbell, JL
我们最近描述了一种新的解旋酶，Dna2解旋酶，这是酵母DNA复制所必需的，我们现在表明酵母FEN-1 （yFEN-1）核酸酶与Dna2解旋酶在遗传和生物化学上相互作用。在酵母中，FEN-1参与DNA复制和修复，yFEN-1的哺乳动物同源物（DNase TV， FEN-1或MF1）参与Okazaki片段成熟。由RAD27/RTH1编码的yFEN-1的过量产生抑制了dna2-1突变体的温度敏感性生长。过量的Dnaa会抑制rad27/rth1 Delta对温度敏感的生长缺陷，Dna2 -1 - rad27/rth1 Delta双突变体是不可存活的，表明该突变具有合成致死性。遗传相互作用可能是由于两种蛋白之间的直接物理相互作用，因为表位标记的yFEN-1和内源性SFEN-1与标记的Dna2共同免疫纯化。对这些数据最简单的解释是，Dna2解旋酶的作用之一与冈崎片段的加工有关。
We have recently described a new helicase, the Dna2 helicase, that is essential for yeast DNA replication, We now show that the yeast FEN-1 (yFEN-1) nuclease interacts genetically and biochemically with Dna2 helicase. FEN-1 is implicated in DNA replication and repair in yeast, and the mammalian homolog of yFEN-1 (DNase TV, FEN-1, or MF1) participates in Okazaki fragment maturation, Overproduction of yFEN-1, encoded by RAD27/RTH1, suppresses the temperature-sensitive growth of dna2-1 mutants. Overproduction of Dnaa suppresses the rad27/rth1 Delta temperature-sensitive growth defect, dna2-1 rad27/rth1 Delta double mutants are inviable, indicating that the mutations are synthetically lethal, The genetic interactions are likely due to direct physical interaction between the two proteins, since both epitope-tagged yFEN-1 and endogenous SFEN-1 coimmuno-purify with tagged Dna2, The simplest interpretation of these data is that one of the roles of Dna2 helicase is associated with processing of Okazaki fragments.