DEMONSTRATION OF LIPIODOL IN PARAFFIN SECTIONS USING A MODIFIED SILVER IMPREGNATION TECHNIQUE
DEMONSTRATION OF LIPIODOL IN PARAFFIN SECTIONS USING A MODIFIED SILVER IMPREGNATION TECHNIQUE
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DOI：
10.1093/ajcp/94.5.585
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发表时间：
1990-11-01
影响因子：
3.5
通讯作者：
LI, MKW
中科院分区：
文献类型：
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作者：
ARNOLD, MM;WALLACE, AC;LI, MKW
为了在石蜡切片中证明血管造影后碘油 (LIP) 在肝细胞癌 (HCC) 中的作用，用硝酸银 (AgNO3) 直接浸渍福尔马林固定的组织块，然后进行常规处理。 LIP 在血窦中表现为黑色小球。研究了来自 13 个血管造影后 LIP HCC 的 94 个组织块和来自 8 个非 LIP HCC 和 4 个脂肪肝的 69 个组织块。 73 个阴性对照中的 72 个以及软组织射线照片 (STR) 上看到的所有阳性块均被正确编码（特异性 98.6%，敏感性 100%）。 LIP 病例的 STR 上的 44 个 LIP 阴性区域中有 26 个区域含有直径小于 10 μm 的少量球体。脂肪变化没有给出积极的读数。因此，改良的 AgNO3 浸渍是一种简单、准确的方法，可在适合肿瘤诊断的高质量石蜡切片中检测 LIP，并且如果应用于血管造影后 LIP、超声引导的肝活检，可以验证活检是否已到达可疑的肿瘤病灶。
To demonstrate postangiographic Lipiodol (LIP) in hepatocellular carcinoma (HCC) in paraffin sections, direct impregnation of formalin-fixed tissue blocks with silver nitrate (AgNO3) was followed by routine processing. LIP appeared as black globules in the sinusoids. Ninety-four tissue blocks from 13 postangiographic LIP HCCs and 69 from 8 non-LIP HCCs and 4 fatty livers were studied. Seventy-two of 73 negative controls and all positive blocks as seen on soft tissue radiographs (STRs) were correctly coded (specificity 98.6%, sensitivity 100%). Twenty-six of the 44 LIP-negative areas on STRs from LIP cases contained scanty globules of less than 10 .mu.m in diameter. Fatty change gave no positive readings. Thus, modified AgNO3 impregnation is a simple, accurate means of detecting LIP in high-quality paraffin sections suitable for tumor diagnosis and, if applied to postangiographic LIP, ultrasonographically guided liver biopsy, can verify that a biopsy has reached a suspected tumor focus.