Silica nanochannel array on co-electrodeposited graphene-carbon nanotubes 3D composite film for antifouling detection of uric acid in human serum and urine samples
Silica nanochannel array on co-electrodeposited graphene-carbon nanotubes 3D composite film for antifouling detection of uric acid in human serum and urine samples
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DOI：
10.1016/j.microc.2023.108632
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发表时间：
2023-03-17
影响因子：
4.8
通讯作者：
Hu, Wei
中科院分区：
文献类型：
--
作者：
Cui, Yu;Zhang, Shiyue;Hu, Wei
本文报道了一种快速、简便的高灵敏检测尿酸(UA)的电化学方法，该方法将含氨基的垂直有序介孔二氧化硅薄膜(NH2-VMSF)与三维石墨烯-碳纳米管(3DG@CNT)复合膜结合在一起。通过一种较为简单的两步可控的电化学沉积方法，将3DG@CNT和NH2-VMSF先后修饰到玻碳电极(GCE)表面。由于3DG@CNT具有较大的比表面积、良好的电催化活性和良好的传质性能，在GCE表面共沉积3DG@CNT不仅可以显著改善电极性能，还可以为进一步功能化提供含氧基团。采用电化学辅助自组装法制备的NH2-VMSF具有均匀的孔径(直径为-2 nm)和带正电的表面，表现出明显的阴离子渗透选择性。由于具有电活性的3DG@CNT内层和静电预浓缩的NH2-VMSF外层，建议的NH2-VMSF/3DG@CNT/GCE对尿酸表现出出色的分析性能，具有宽的线性范围(0.1类似于50微米)、高灵敏度(1.66微米/微米-1)和低检测下限(6.8纳米)。此外，考虑到NH2-VMSF固有的防污性能，实现了对人血清和尿样中尿酸的直接分析，获得了满意的回收率。
The present work reports a rapid and convenient electrochemical method for highly sensitive detection of uric acid (UA) by integration of vertically-ordered mesoporous silica film bearing amino groups (NH2-VMSF) and three-dimensional graphene-carbon nanotubes (3DG@CNT) composite film. 3DG@CNT and NH2-VMSF are successively modified onto the glassy carbon electrode (GCE) surface through a rather simple two-step controllable electrochemical deposition method. Arising from the large specific surface area, excellent electro-catalytic activity, and favorable mass transfer, 3DG@CNT co-electrodeposited onto the GCE surface could not only significantly improve the electrode performance, but also provide oxygen-containing groups for further functionalization. NH2-VMSF prepared by co-condensation approach using electrochemically assisted self-assembly method, possesses uniform pore size (-2 nm in diameter) and positively charged surface, showing pronounced anionic permselectivity. Thanks to the electroactive 3DG@CNT inner layer and electrostatically preconcentrated NH2-VMSF outer layer, the proposed NH2-VMSF/3DG@CNT/GCE displays outstanding analytical performance towards UA with a wide linear range (0.1 similar to 50 mu M), a high sensitivity (1.66 mu A.mu M-1) and a low limit of detection (6.8 nM). Furthermore, considering the inherent anti-fouling property of NH2-VMSF, direct analysis of UA in human serum and urine samples is achieved with satisfactory recoveries.