BLOOD-BRAIN-BARRIER PERMEABILITY DURING CUPRIZONE-INDUCED DEMYELINATION - IMPLICATIONS FOR THE PATHOGENESIS OF IMMUNE-MEDIATED DEMYELINATING DISEASES
BLOOD-BRAIN-BARRIER PERMEABILITY DURING CUPRIZONE-INDUCED DEMYELINATION - IMPLICATIONS FOR THE PATHOGENESIS OF IMMUNE-MEDIATED DEMYELINATING DISEASES
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DOI：
10.1016/0022-510x(87)90055-4
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发表时间：
1987-04-01
影响因子：
4.4
通讯作者：
LUDWIN, SK
中科院分区：
文献类型：
--
作者：
BAKKER, DA;LUDWIN, SK
研究人员对喂食铜宗的小鼠小脑上脚的血管进行了研究，以了解蛋白质渗漏到实质中的情况。通过使用辣根过氧化物酶的示踪方法和使用外渗血清蛋白的抗血清的免疫化学方法来确定脱髓鞘的各个阶段的血脑屏障的状态，并与三种阳性和阴性对照条件进行比较。结果显示，在脱髓鞘发展过程中，没有证据表明铜宗小鼠的内皮下基底膜或血管外空间有明显的蛋白质渗漏。与之前报道的免疫介导的脱髓鞘疾病的血脑屏障改变相比，在 Cuprizone 动物中发现血脑屏障明显缺乏损伤，这证实了这样的理论：这些改变不是任何脱髓鞘过程的非特异性关联，而是在免疫介导的脱髓鞘中具有主要发病机制。
Blood vessels in the superior cerebellar peduncles were studied in Cuprizone-fed mice for leakage of proteins into the parenchyma. The status of the blood-brain barrier was determined at various stages of demyelination both by tracer methods using horseradish peroxidase and immunochemically using antisera to extravasated serum proteins and was compared to three positive and negative control conditions. The results showed no evidence of significant protein leakage into the subendothelial basement membrane or extravascular space in Cuprizone mice, during the development of demyelination. The apparent lack of damage to the blood-brain barrier found in Cuprizone animals as compared to the blood-brain barrier alterations previously reported for immune-mediated demyelinating diseases, confirms the theory that these alterations are not a non-specific association of any demyelinating process, but are of primary pathogenetic importance in immune-mediated demyelination.