Human papillomavirus in high- and low-risk areas of oesophageal squamous cell carcinoma in China.
Human papillomavirus in high- and low-risk areas of oesophageal squamous cell carcinoma in China.
复制标题
中国食管鳞状细胞癌的高风险区域中的人乳头瘤病毒。
DOI：
10.1038/sj.bjc.6603765
复制
发表时间：
2007-05-21
影响因子：
8.8
通讯作者：
Akiba, S
中科院分区：
文献类型：
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作者：
Shuyama, K;Castillo, A;Aguayo, F;Sun, Q;Khan, N;Koriyama, C;Akiba, S
为了研究人乳头瘤病毒（HPV）在食管鳞状细胞癌（ESCC）发展中的潜在作用，我们使用SPF10引物进行PCR，或使用SPF10引物进行PCR，检测了从中国不同ESCC发病率的两个地区（即甘肃（n = 26）和山东（n = 33））收集的石蜡包埋的ESCC组织中HPV DNA的存在情况。 GP5+/GP6+引物结合Southern印迹杂交。 HPV基因型通过INNO-LiPA HPV基因分型试剂盒测定。甘肃的食管鳞癌发病率远高于山东的 17 例（65%），山东有 2 例 HPV 阳性（6%）。 HPV 阳性样本中分别检测到 79% 和 16% 的 HPV 基因型 16 和 18。实时 PCR 分析表明，所有 HPV-16 阳性样本中都存在整合形式的 HPV DNA，但其病毒载量估计仅为 <1-2 个拷贝细胞−1。我们无法通过免疫染色在任何 HPV-16 阳性样本中检测到 HPV 16/18 E6 蛋白表达。 p16INK4a 和 p53 表达均与 ESCC 中 HPV 的存在无关。似乎有必要进一步研究 HPV 在 ESCC 中可能的病因学作用。
To examine the potential roles of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (ESCC) development, we examined the presence of HPV DNA in paraffin-embedded ESCC tissues collected from two areas with different ESCC incidence rates in China, that is, Gansu (n=26) and Shandong (n=33), using PCR with SPF10 primers, or PCR with GP5+/GP6+ primers combined with Southern blot hybridisation. HPV genotype was determined by the INNO-LiPA HPV genotyping kit. HPV DNA was detected in 17 cases (65%) in Gansu, where ESCC incidence is much higher than in Shandong, where HPV was positive in two samples (6%). HPV genotypes 16 and 18 were detected in 79 and 16% of HPV-positive samples, respectively. Real-time PCR analysis suggested the presence of integrated form of HPV DNA in all the HPV-16-positive samples, but its viral load was estimated to be only <1–2 copies cell−1. We could not detect HPV 16/18 E6 protein expression by immunostaining in any of the HPV-16-positive samples. Neither p16INK4a nor p53 expression was related to HPV presence in ESCCs. Further studies seem warranted to examine the possible aetiological roles of HPV in ESCC.