Organization of the mouse ASGR1 gene encoding the major subunit of the hepatic asialoglycoprotein receptor.
Organization of the mouse ASGR1 gene encoding the major subunit of the hepatic asialoglycoprotein receptor.
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DOI：
10.1016/s0378-1119(99)00493-x
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发表时间：
2000-01
期刊：
影响因子：
3.5
通讯作者：
S. Soukharev;W. Berlin;J. Hanover;B. Bethke;B. Sauer
中科院分区：
文献类型：
--
作者：
S. Soukharev;W. Berlin;J. Hanover;B. Bethke;B. Sauer
肝脏脱唾液酸糖蛋白受体是第一个被识别的哺乳动物凝集素，并且三十年来一直是深入研究的主题。然而，这种主要肝内吞受体的确切生物学作用仍然难以捉摸。我们在此描述了该受体 (ASGR1) 主要亚基的小鼠基因以及上游 5' 区域的 3.5kb 的特征。该基因包含八个编码外显子，肝脏中的主要转录物显示单个非编码 5' 外显子。次要肝转录本在主要起始上游 435bp 处起始，并包含额外的 5' 非编码外显子和内含子。 ASGR1 的最小 600bp 近端区域在体外 HepG2 细胞中表现出肝特异性启动子活性。这些结果为更详细地研究哺乳动物肝脏脱唾液酸糖蛋白受体的功能作用提供了基础。
The hepatic asialoglycoprotein receptor was the first of the mammalian lectins to be recognized and has been the subject of intense investigation for three decades. Yet, the precise biological role of this major hepatic endocytic receptor has remained elusive. We describe here the characterization of the mouse gene for the major subunit of this receptor (ASGR1) along with 3.5kb of the upstream 5′ region. The gene comprises eight coding exons, with the major transcript in liver displaying a single non-coding 5′ exon. A minor hepatic transcript initiates 435bp upstream of the major start and includes an additional 5′ non-coding exon and intron. A minimal 600bp proximal region of ASGR1 exhibits hepatic-specific promoter activity in HepG2 cells in vitro. These results provide the basis for more detailed genetic studies on the functional role of the hepatic asialoglycoprotein receptor in mammals.