Filamentation Involves Two Overlapping, but Distinct, Programs of Filamentation in the Pathogenic Fungus Candida albicans.
Filamentation Involves Two Overlapping, but Distinct, Programs of Filamentation in the Pathogenic Fungus Candida albicans.
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DOI：
10.1534/g3.117.300224
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发表时间：
2017-11-06
期刊：
影响因子：
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通讯作者：
Blankenship JR
中科院分区：
文献类型：
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作者：
Azadmanesh J;Gowen AM;Creger PE;Schafer ND;Blankenship JR
人类致病性真菌白色念珠菌在酵母样和丝状生长形式之间切换的能力长期以来一直与发病机制有关。许多环境条件，包括高温生长、营养限制和暴露于血清，都可以触发这种形态转换，并经常在体外模型中用于鉴定在丝化中起作用的基因。先前的研究表明，各种体外模型在成丝的遗传要求和对不同成丝诱导介质的转录反应方面存在差异，但这些差异尚未得到详细分析。我们比较了10个体外纤维模型，发现模型系统之间存在广泛的遗传和转录组差异。通过比较分析，我们发现了新的与媒介无关的丝化遗传要求以及核心丝化转录谱。我们的数据还表明，物理环境驱动白色念珠菌不同的丝化程序，这对体内丝化具有重要意义。
The ability of the human pathogenic fungus Candida albicans to switch between yeast-like and filamentous forms of growth has long been linked to pathogenesis. Numerous environmental conditions, including growth at high temperatures, nutrient limitation, and exposure to serum, can trigger this morphological switch and are frequently used in in vitro models to identify genes with roles in filamentation. Previous work has suggested that differences exist between the various in vitro models both in the genetic requirements for filamentation and transcriptional responses to distinct filamentation-inducing media, but these differences had not been analyzed in detail. We compared 10 in vitro models for filamentation and found broad genetic and transcriptomic differences between model systems. The comparative analysis enabled the discovery of novel media-independent genetic requirements for filamentation as well as a core filamentation transcriptional profile. Our data also suggest that the physical environment drives distinct programs of filamentation in C. albicans, which has significant implications for filamentation in vivo.