Nuclear morphology measurements using Fourier domain low coherence interferometry
Nuclear morphology measurements using Fourier domain low coherence interferometry
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DOI：
10.1364/opex.13.004693
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发表时间：
2005-06-13
期刊：
影响因子：
3.8
通讯作者：
Wax, A
中科院分区：
文献类型：
--
作者：
Graf, RN;Wax, A
We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical system uses a white light source in a modified Michelson interferometer and a spectrograph for detection of the mixed signal and reference fields. Depth resolution is obtained from the Fourier transform of the measured spectrum which provides the axial spatial cross-correlation between the signal and reference fields. The spectral dependence of scattering by the samples is determined by windowing the spectrum to measure the scattering amplitude as a function of wavenumber. We present evidence that fLCI accurately measures the longitudinal profile of cell nuclei rather than the transverse profile. (C) 2005 Optical Society of America.
