Local Dkk1 crosstalk from breeding ornaments impedes regeneration of injured male zebrafish fins.
Local Dkk1 crosstalk from breeding ornaments impedes regeneration of injured male zebrafish fins.
复制标题
DOI：
10.1016/j.devcel.2013.08.015
复制
发表时间：
2013-10-14
影响因子：
11.8
通讯作者：
Poss, Kenneth D.
中科院分区：
文献类型：
--
作者：
Kang, Junsu;Nachtrab, Gregory;Poss, Kenneth D.
精确的时空调节信号激活剂和抑制剂可以帮助限制发育过程中相邻组织之间的串扰形态，稳态和再生。在这里，我们发现，分泌的Wnt抑制剂Dkk1b是丰富的雄激素调节的表皮结节（ET）的成年雄性斑马鱼胸鳍表面上的致密区域。高速摄像和截肢实验表明，胸鳍和其ET用于男性产卵。ET的形成和旺盛的周转涉及Dkk1b的诱导和维持，而Dkk1b通常在截肢损伤后的再生芽基中受到限制。当通过含ET区域发生截肢时，Dkk1b富集的伤口表皮形成，芽基形成被破坏，损害再生。因此，关键繁殖装饰物的稳态信号可以干扰损伤激活的组织再生。我们的研究结果有助于解释斑马鱼的性二态鳍再生，并对再生潜力如何随着发育过程或物种进化而下降产生影响。
Precise spatiotemporal regulation of signaling activators and inhibitors can help limit developmental crosstalk between neighboring tissues during morphogenesis, homeostasis, and regeneration. Here, we find that the secreted Wnt inhibitor Dkk1b is abundantly produced by dense regions of androgen-regulated epidermal tubercles (ET) on the surfaces of adult male zebrafish pectoral fins. High-speed videos and amputation experiments reveal that pectoral fins and their ET are employed for male spawning. Formation and vigorous turnover of ET involve Dkk1b induction and maintenance, whereas Dkk1b is typically restricted from the regeneration blastema after amputation injury. When amputation occurs through an ET-containing region, a Dkk1b-enriched wound epidermis forms and blastema formation is disrupted, compromising regeneration. Thus, homeostatic signaling by key breeding ornaments can interfere with injury-activated tissue regeneration. Our findings help explain sexually dimorphic fin regeneration in zebrafish, and have implications for how regenerative potential might decline as development progresses or during species evolution.
登录
查看更多内容
DOI：
10.1007/978-1-4419-7037-4_13
发表时间：
2010-01-01
期刊：
CELL BIOLOGY OF STEM CELLS
影响因子：
--
作者：
Antos, Christopher L.;Tanaka, Elly M.
通讯作者：
Tanaka, Elly M.
影响因子：
11.8
作者：
Lin, Gufa;Chen, Ying;Slack, Jonathan M. W.
通讯作者：
Slack, Jonathan M. W.
影响因子：
8.8
作者：
GHADIALLY, F N;WHITELEY, H J
通讯作者：
WHITELEY, H J
影响因子：
4.6
作者：
Chablais, Fabian;Jazwinska, Anna
通讯作者：
Jazwinska, Anna
影响因子：
9.2
作者：
Jazwinska, Anna;Badakov, Rossen;Keating, Mark T.
通讯作者：
Keating, Mark T.