Luteoloside Protects the Uterus from Staphylococcus aureus-Induced Inflammation, Apoptosis, and Injury
Luteoloside Protects the Uterus from Staphylococcus aureus-Induced Inflammation, Apoptosis, and Injury
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木犀草苷保护子宫免受金黄色葡萄球菌引起的炎症、细胞凋亡和损伤、炎症
DOI：
10.1007/s10753-018-0814-7
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发表时间：
2018-10-01
期刊：
影响因子：
5.1
通讯作者：
Deng, Ganzhen
中科院分区：
文献类型：
--
作者：
Wang, Xiaoyan;Yuan, Ting;Deng, Ganzhen
木犀草苷是一种从几种天然草药中提取的类黄酮，具有抗微生物和抗炎症的特性。本研究主要探讨木犀草苷在金黄色葡萄球菌所致子宫内膜炎中的抗炎作用机制。组织病理学观察和髓过氧化物酶（MPO）活性显示木樨草苷能保护子宫免受金黄色葡萄球菌诱导的损伤，改善炎症细胞的浸润。定量PCR （qPCR）和ELISA分析也显示木犀草苷在体内和体外均能降低促炎细胞因子TNF-、IL-1和IL-6的表达，提高抗炎细胞因子IL-10的表达。western blot分析显示木犀草苷抑制TLR2和IL-8的表达，抑制IB和NF-B p65的磷酸化。木本草苷抑制子宫内膜上皮细胞（EECs）凋亡，抑制p53磷酸化，降低金黄色葡萄球菌诱导的caspase-3表达。此外，本研究表明木犀草苷抑制Bax的表达，增加Bcl-2的表达。这些结果表明木犀草苷通过抑制TLR2和NF-B信号通路具有抗炎作用，在金黄色葡萄球菌诱导的子宫内膜炎中具有抗凋亡作用，可能对临床治疗金黄色葡萄球菌诱导的炎症具有重要价值。
Luteoloside is a flavonoid extracted from several natural herbs that exhibits anti-microbial and anti-inflammation properties. Our study mainly identified the anti-inflammatory mechanism of action of luteoloside in Staphylococcus aureus-induced endometritis. Histopathological observations and myeloperoxidase (MPO) activity showed that luteoloside could protect the uterus from S. aureus-induced damage and ameliorate the infiltration of inflammatory cells. Quantitative PCR (qPCR) and ELISA analysis also revealed that luteoloside could decrease the expression of the pro-inflammatory cytokines TNF-, IL-1, and IL-6 and increase the expression of the anti-inflammatory cytokine IL-10 both in vivo and in vitro. However, western blot analysis revealed that luteoloside inhibited the expression of TLR2 and IL-8 and inhibited the phosphorylation of IB and NF-B p65. Moreover, luteoloside inhibited the apoptosis of endometrial epithelial cells (EECs), suppressed the phosphorylation of p53, and decreased the expression of caspase-3 induced by S. aureus. Furthermore, this study showed that luteoloside inhibited the expression of Bax but increased the expression of Bcl-2. These results indicate that luteoloside has anti-inflammatory properties by inhibiting the TLR2 and NF-B signaling pathways and plays an anti-apoptotic role in S. aureus-induced endometritis, which may be valuable for the clinical treatment of S. aureus-induced inflammation.