Enzyme electrode formed by evaporative concentration and its performance characterization
Enzyme electrode formed by evaporative concentration and its performance characterization
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DOI：
10.1016/j.bios.2007.02.004
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发表时间：
2007-06-15
影响因子：
12.6
通讯作者：
Suzuki, Hiroaki
中科院分区：
文献类型：
--
作者：
Hashimoto, Masatoshi;Sakamoto, Naohisa;Suzuki, Hiroaki
高浓度的固定化酶层上形成一个小的工作电极，和电极的行为作为电流传感器进行了检查。为此，通过使用聚四氟乙烯（PTFE）珠在除敏感区域之外的区域中形成超疏水层。将一小滴含有葡萄糖氧化酶（GOD）和牛血清白蛋白（BSA）的酶溶液置于敏感区域，通过蒸发浓缩，并与戊二醛交联。在单位面积酶活性相同的情况下，工作电极越小，电流密度越大。此外，电流密度随着较高的酶载量增加至极限值。此外，校准图的线性范围扩大到更高的葡萄糖浓度。将该方法制备的酶电极组装在微流道中。与具有相同单位面积酶活性的大酶电极相比，较小的电极显示出电流密度的显著增加和流量依赖性的降低。通过缩小流道和增加电极数量可以提高转换效率，这与将大电极放置在浅流道中相当。(c)2007 Elsevier B.V.保留所有权利。
A highly concentrated immobilized enzyme layer was formed on a small working electrode, and the behavior of the electrode as an amperometric sensor was examined. To this end, a super-hydrophobic layer was formed in an area other than the sensitive area by using polytetrafluoroethylene (PTFE) beads. A small droplet of an enzyme solution containing glucose oxidase (GOD) and bovine serum albumin (BSA) was placed on the sensitive area, concentrated by evaporation, and crosslinked with glutaraldehyde. With the same enzyme activity per unit area, the current density increased with smaller working electrodes. Also, the current density increased with higher enzyme loadings up to a limiting value. In addition, the linear range of the calibration plot was expanded to higher glucose concentrations. The enzyme electrode fabricated by the novel method was incorporated in a micro-flow channel. Compared with large enzyme electrodes with the same enzyme activity per unit area, smaller electrodes showed a significant increase in the current density and a decrease in the flow dependence. The conversion efficiency could be improved by narrowing the flow channel and increasing the number of electrodes, which was comparable with a large electrode placed in a shallow flow channel. (c) 2007 Elsevier B.V. All rights reserved.