DESCRIPTION OF A SEROLOGICAL REACTION CHARACTERISTIC OF POLYMYOSITIS
DESCRIPTION OF A SEROLOGICAL REACTION CHARACTERISTIC OF POLYMYOSITIS
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DOI：
10.1016/0090-1229(76)90145-8
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
MATTIOLI, M
中科院分区：
文献类型：
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作者：
REICHLIN, M;MATTIOLI, M
许多多发性肌炎血清与小牛胸腺提取物中的抗原产生补体结合反应。由于该系统的沉淀反应特征较弱且不稳定，因此无法通过琼脂凝胶双扩散的Ouchterlony方法对反应的特异性进行分类。为了鉴定该反应的特异性，开发了以下方法。任何多发性肌炎血清的木瓜蛋白酶消化将血清的补体固定能力转化为这些多发性肌炎血清的反应特征的有效和特异性抑制剂。木瓜蛋白酶消化的血清中含有多种抗体的抗原小牛胸腺提取物的各种结缔组织疾病的患者未能抑制补体结合反应的多发性肌炎血清的特点。小牛胸腺提取物中的这种抗原的抗体似乎对多发性肌炎综合征患者，特别是皮肌炎患者最具特异性。
Numerous polymyositis sera gave complement fixation reactions with an antigen in calf thymus extract. Because of the weak and inconstant precipitin reactions characteristic of this system, the specificity of the reaction could not be classified by the Ouchterlony method of double diffusion in agar gel. To identify the specificity of this reaction, the following method was developed. Papain digestion of any of the polymyositis sera converted the complement fixing capacities of the sera to potent and specific inhibitors of the reaction characteristic of these polymyositis sera. Papain digestion of sera from patients with a variety of connective tissue diseases containing a multiplicity of antibodies to antigens in calf thymus extract failed to inhibit the complement fixation reaction characteristic of the polymyositis sera. Antibodies to this antigen in calf thymus extract appear most specific for patients with polymyositis syndromes, particularly those with dermatomyositis.