Comparative studies on dietary protein requirements of juvenile and on‐growing gibel carp (Carassius auratus gibelio) based on fishmeal‐free diets
Comparative studies on dietary protein requirements of juvenile and on‐growing gibel carp (Carassius auratus gibelio) based on fishmeal‐free diets
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DOI：
10.1111/anu.12156
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发表时间：
2015-06
影响因子：
3.5
通讯作者：
W. Ye;Dong Han;Xiyu Zhu;Yunxia Yang;Junyan Jin;S. Xie
中科院分区：
文献类型：
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作者：
W. Ye;Dong Han;Xiyu Zhu;Yunxia Yang;Junyan Jin;S. Xie
为研究异育银鲫（Carassius auratus gibelio var. CAS III）幼鱼（试验1为3.18g）和正生长期（试验2为87.1g）的蛋白质需用量，进行了两项试验。以大豆浓缩蛋白（SPC）和酪蛋白为蛋白质源，配制6种日粮蛋白质含量为250 ~ 500gkg（-1）的等能饲粮。结果表明：在试验1中，饲粮蛋白质从250 ~ 400gkg（-1）增加时增重增加，在400 ~ 500gkg(-1) CP时降低；在试验2中，饲粮蛋白质从250 ~ 350gkg（-1）增加时增重增加，在350 ~ 500gkg(-1) CP时保持不变。随着饲粮蛋白质的增加，试验1的饲料系数（FCR）降低，蛋白质保留效率（PRE）降低，试验2不受影响。蛋白质表观消化系数（ADC(p)）随饲粮蛋白质含量的增加而升高。胰蛋白酶活性在幼鱼中随饲料蛋白质的增加而增加，而在生长中的鱼中不受影响。肝脏丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）活性随饲粮蛋白质含量的增加而升高。WG的折线和二次回归估计，幼鱼最大生长所需的饲料蛋白质约为402 ~ 427gkg(-1)，未生长的吉伯鱼约为337 ~ 418gkg（-1）。
Two trials were conducted to investigate protein requirements of juvenile (3.18g in Trial 1) and on-growing (87.1g in Trial 2) gibel carp, Carassius auratus gibelio var. CAS III. Six isoenergetic diets containing 250-500gkg(-1) dietary protein were formulated using soy protein concentrate (SPC) and casein as protein sources. The results showed that weight gain (WG) increased when dietary protein increased from 250 to 400gkg(-1) and decreased at 400 to 500gkg(-1) CP in Trial 1, while WG increased when dietary protein increased from 250 to 350gkg(-1) and kept constant at 350 to 500gkg(-1) CP in Trial 2. With increasing dietary protein, feed conversion ratio (FCR) decreased, while protein retention efficiency (PRE) decreased in Trial 1 and was not affected in Trial 2. Apparent digestibility coefficient of protein (ADC(p)) increased with increasing dietary protein in two trails. Trypsin activity increased with dietary protein in the juveniles and was not affected in on-growing fish. Hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities increased with dietary protein. Broken-line and quadratic regression of WG estimated that dietary protein requirements for maximum growth were about 402-427gkg(-1) for the juvenile and 337-418gkg(-1) for on-growing gibel carp.