Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells
Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells
复制标题
DOI：
10.1016/j.neulet.2009.02.069
复制
发表时间：
2009-04-24
影响因子：
2.5
通讯作者：
Taniguchi, Kazuyuki
中科院分区：
文献类型：
--
作者：
Yamamoto, Yoshio;Hatakeyama, Taku;Taniguchi, Kazuyuki
Trek属于串联孔结构域K(+)通道的一个亚家族，由三个亚单位组成：Trek-1、Trek-2和TRAAK。我们观察了TREK-1、TREK-2和TRAAK免疫反应神经元在大鼠三叉神经感觉神经元中的分布。在三叉神经节，Trek-1、Trek-2和TRAAK的阳性神经元分别占31%、43%和60%。Trek-1、Trek-2和TRAAK免疫反应阳性神经元的平均大小分别为447+/-185、445+/-23和492+/-12 mm(2)。此外，Trek通道与阳离子Trp通道、TRPV1、TRPV2和TRPM8共定位。Trek-1免疫反应阳性神经元与TRPV1(57%)、TRPV2(11%)和TRPM8(33%)共定位。TRPV1(33%)、TRPV2(9%)和TRPM8(19%)与Trek-2免疫反应阳性神经元共存。TRAAK免疫反应阳性神经元与TRPV1(47%)、TRPV2(10%)和TRPM8(22%)共定位。结果表明，在一些小的三叉神经节神经元中，Trek-1、Trek-2和TRAAK通道与温度敏感的Trp通道共存。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
TREK belongs to a subfamily of tandem pore domain K(+) channels, and consists of three subunits, TREK-1, TREK-2 and TRAAK. We examined the distribution of TREK-1, TREK-2 and TRAAK immunoreactive neurons in rat trigeminal sensory neurons. In the trigeminal ganglia, 31%, 43% and 60% of neurons were immunoreactive for TREK-1, TREK-2 and TRAAK, respectively. Mean sizes of TREK-1, TREK-2 and TRAAK immunoreactive trigeminal ganglion neurons were 447 +/- 185, 445 +/- 23 and 492 +/- 12 mm(2), respectively. Furthermore, TREK channels were colocalized with cationic TRP channels, TRPV1, TRPV2 and TRPM8. TREK-1 immunoreactive neurons were colocalized with TRPV1 (57%), TRPV2 (11%) and TRPM8 (33%). TREK-2-immunoreactive neurons were colocalized with TRPV1 (33%), TRPV2 (9%) and TRPM8 (19%). TRAAK immunoreactive neurons were colocalized with TRPV1 (47%), TRPV2 (10%) and TRPM8 (22%). The present results revealed that TREK-1, TREK-2 and TRAAK channels colocalized with thermosensitive TRP channels in some small trigeminal ganglion neurons. (C) 2009 Elsevier Ireland Ltd. All rights reserved.