PriA-directed assembly of a primosome on D loop DNA
PriA-directed assembly of a primosome on D loop DNA
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DOI：
10.1074/jbc.274.35.25033
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发表时间：
1999-08-27
影响因子：
4.8
通讯作者：
Marians, KJ
中科院分区：
文献类型：
--
作者：
Liu, J;Marians, KJ
携带priA零突变的大肠杆菌菌株长期诱导SOS反应，在同源重组、紫外线损伤DNA修复、双链断裂修复以及诱导和组成稳定DNA复制方面存在缺陷。这导致了PriA在由同源重组机制形成的D环上指导复制叉组装的建议。PriA特异性识别和结合D环DNA的证明支持了这一假设。使用DNA足迹作为分析，我们在这里表明，PriA也指导了D环DNA上phi x174型原体的组装。将一个完整的前原体装载到D环DNA上的能力是复制叉组装的必要步骤。
Escherichia coli strains carrying null mutations in priA are chronically induced for the SOS response and are defective in homologous recombination, repair of UV damaged DNA, double-strand break repair, and both induced and constitutive stable DNA replication. This led to the proposal that PriA directed replication fork assembly at D loops formed by the homologous recombination machinery. The demonstration that PriA specifically recognized and bound D loop DNA supported this hypothesis. Using DNA footprinting as an assay, we show here that PriA also directs the assembly of a phi X174-type primosome on D loop DNA. The ability to load a complete primosome on D loop DNA is a step necessary for replication fork assembly.