Expression of Notch signalling-related genes in normal and differentiating rat dental pulp cells
Expression of Notch signalling-related genes in normal and differentiating rat dental pulp cells
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DOI：
10.1111/j.1747-4477.2009.00188.x
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发表时间：
2010-08-01
影响因子：
1.6
通讯作者：
Suda, Hideaki
中科院分区：
文献类型：
--
作者：
Sun, Hantang;Kawashima, Nobuyuki;Suda, Hideaki
Notch信号传导对胚胎发育和成人的各种过程至关重要。Hey 1是一种重要的Notch信号成分，在成牙本质细胞分化中可能发挥的作用在这项研究中进行了评估。将来源于5周龄Wistar大鼠上门牙的原代培养牙髓细胞置于添加10%胎牛血清（FBS）、抗坏血酸（AA）和β-甘油磷酸盐（β-GP）的Eagle最低必需培养基的α修饰培养基中，含或不含地塞米松，并在涂有IA型胶原蛋白的培养皿上培养7天。采用常规和实时荧光定量PCR技术检测Notch相关基因和牙本质涎磷蛋白的表达，作为成牙本质细胞分化的标志。与对照组相比，AA + β-GP组牙本质涎磷蛋白和Hey 1的表达分别显著增加和减少。这些结果表明Hey 1可能是成牙本质细胞分化的负调控因子。
Notch signalling is of fundamental importance to various processes during embryonic development and in adults. The possible role of Hey1, an important Notch signalling component, in odontoblast differentiation was evaluated in this study. Primary cultured dental pulp cells, derived from upper incisors of 5-week-old Wistar rats, were placed in alpha-modification of Eagle's minimal essential medium supplemented with 10% Fetal Bovine Serum (FBS), and ascorbic acid (AA) and beta-glycerophosphate (beta-GP), with or without dexamethasone, and cultured on dishes coated with collagen type IA for 7 days. Conventional and real-time Polymerase Chain Reaction (PCR) was performed to determine the expression of Notch-related genes and dentin sialophosphoprotein as a marker of odontoblast differentiation. Dentin sialophosphoprotein and Hey1 expression was significantly increased and decreased in the presence of AA + beta-GP compared with controls, respectively. These findings suggest that Hey1 may be a negative regulator in odontoblast differentiation.