A study of peripheral tolerance through embryonic grafts of the bursal epithelial rudiment between MHC-distinct chick embryos.
A study of peripheral tolerance through embryonic grafts of the bursal epithelial rudiment between MHC-distinct chick embryos.
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通过 MHC 不同鸡胚之间法氏囊上皮雏形的胚胎移植进行外周耐受性的研究。
DOI：
10.1093/intimm/6.6.795
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发表时间：
1994
影响因子：
4.4
通讯作者：
C. Corbel
中科院分区：
文献类型：
--
作者：
C. Martin;M. Belo;N. L. Le Douarin;C. Corbel
我们在此报告，即使在发育早期，即在宿主免疫系统开始发育之前，不同的器官移植诱导宿主耐受的能力并不相同。我们在胚胎5岁时将法氏囊的上皮间充质雏形移植到组织不相容的鸡胚胎之间，发现移植通常由宿主的造血细胞定植，最终产生正常的B细胞。移植的法氏囊在出生后可以耐受几周(或=4-8)，但在所有情况下都会被一种免疫机制排斥，这种免疫机制不能被4-5个月龄时发生的生理退化过程同化。此外，即使在出生后的第一个月，法氏囊仍然健康，相同MHC单倍型的皮肤移植也会立即被排斥。这些观察结果与同种异体肢芽移植的结果形成对比，同种异体肢体芽移植在出生后可以永久耐受，尽管方式并不完美。此外，我们还表明，就像从鹌鹑和鸡胚胎中移植法氏囊和肢芽的异种移植一样，法氏囊MHC单倍型的同种异体原位移植到胸腺上皮诱导了法氏囊的耐受性。肢体芽、法氏囊甚至胸腺残基的异种和异基因胚胎移植的一个共同点是，由于在体外单向宿主-供者混合白细胞培养中总是获得增殖反应，它们都没有诱导完全的耐受状态。
We report here that different organ grafts are not equally competent to induce tolerance of the host even if they are performed early in development, i.e. before the host's immune system has started to develop. We have grafted the epithelio-mesenchymal rudiment of the bursa of Fabricius between histoincompatible chicken embryos at E5 and found that the transplant is normally colonized by hemopoietic cells from the host and that a normal contingent of B cells is eventually produced. The grafted bursa is tolerated after birth for a few weeks (> or = 4-8) but is in all cases rejected by an immune mechanism which cannot be assimilated to the physiological involution process which occurs at 4-5 months of age. Moreover, even in the first month after birth, when the bursa is still healthy, skin grafts of the same MHC haplotype are promptly rejected. These observations are in contrast with the outcome of allogeneic limb bud grafts which are permanently tolerated after birth although in an unperfect manner. We show in addition that, as was the case in xenogeneic grafts of limb buds and bursas of Fabricius from quail and chick embryos, the allogeneic in situ graft to thymic epithelium of the MHC haplotype of the bursal implant induces tolerance of the bursa. One common point of xenogeneic and allogeneic embryonic grafts of limb bud, bursa and even thymic rudiments is that none of them induced a complete state of tolerance, since proliferation responses were always obtained in vitro in one-way host-donor mixed leucocyte cultures.