Clonal dissemination of Vibrio parahaemolyticus displaying similar DNA fingerprint but belonging to two different serovars (O3:K6 and O4:K68) in Thailand and India
Clonal dissemination of Vibrio parahaemolyticus displaying similar DNA fingerprint but belonging to two different serovars (O3:K6 and O4:K68) in Thailand and India
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DOI：
10.1017/s0950268899004070
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发表时间：
2000-08-01
影响因子：
4.2
通讯作者：
Nair, GB
中科院分区：
文献类型：
--
作者：
Chowdhury, NR;Chakraborty, S;Nair, GB
对印度加尔各答住院病人中副溶血性弧菌感染的主动监测显示，1998年3月O4:K68血清型首次出现，同时O3:K6血清型继续占主导地位。据报道，属于这两种血清型的毒株具有大流行的潜力。采用不同的分子技术对来自印度和泰国的O3:K6和O4:K68菌株的基因组进行了检测，以确定它们的亲缘关系。来自加尔各答和曼谷的O3:K6和O4:K68菌株携带tdh基因，而不携带trh基因。对这两种血清型的代表性菌株进行了核糖分型和任意引物聚合酶链反应（AP-PCR）鉴定，结果表明两株分离物具有相同的核糖分型和DNA指纹图谱。对来自加尔各答和泰国的O3:K6和O4:K68分离株进行脉冲场凝胶电泳（PFGE）分析，得到了几乎相似的限制性片段长度多态性（RFLP）模式。NotI RFLP的系统发育分析表明，O3:K6和O4:K68形成了一个相似度为78 ~ 91%的聚类，这表明在同一时间段分离的两个不同的血清型之间存在着密切的遗传关系，但它们来自不同的地理区域。而非o3:K6和非o4:K68则表现出不同的核糖型、AP-PCR和PFGE模式。
Active surveillance of Vibrio parahaemolyticus infection among hospitalized patients in Calcutta, India, showed the appearance of the O4:K68 serovar for the: first time in March 1998 alongside the continued predominant incidence of the O3:K6 serovar. Strains belonging to both these serovars have been reported to possess pandemic potential. The genomes of O3:K6 and O4:K68 strains and for comparison, non-O3:K6 and non-O4:K68 strains isolated from two different countries, India and Thailand, were examined by different molecular techniques to determine their relatedness. The O3:K6 and O4:K68 strains from Calcutta and Bangkok carried the tdh gene but not the trh gene. Characterization of representative strains of these two serovars by ribotyping and by arbitrarily primed-polymerase chain reaction (AP-PCR) showed that the isolates had identical ribotype and DNA fingerprint. Pulsed-field gel electrophoresis (PFGE) performed with the same set of strains yielded nearly similar restriction fragment length polymorphism (RFLP) patterns for the O3:K6 and O4:K68 isolates from Calcutta and Thailand. Phylogenetic analysis of the NotI RFLP showed that the O3:K6 and O4:K68 strains formed a cluster with 78-91% similarity thus indicating close genetic relationship between the two different serovars isolated during the same time-frame but from widely separated geographical regions. The non-O3:K6 and non-O4:K68, in contrast, showed different ribotype, AP-PCR and PFGE patterns.