Subcutaneous and omental fat expression of adiponectin and leptin in women with polycystic ovary syndrome
Subcutaneous and omental fat expression of adiponectin and leptin in women with polycystic ovary syndrome
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DOI：
10.1016/j.fertnstert.2007.03.085
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发表时间：
2008-03-01
影响因子：
6.7
通讯作者：
Lobo, Rogerio
中科院分区：
文献类型：
--
作者：
Carmina, Enrico;Chu, Micheline C.;Lobo, Rogerio
目的：探讨脂联素和瘦素在多囊卵巢综合征(PCOS)患者内脏脂肪和SC脂肪中的表达。设计：前瞻性临床试验。地点：学术医学。患者(S)：多囊卵巢综合征患者和对照妇女。干预(S)：手术活检内脏(大网膜)和皮下(SC)脂肪组织，空腹血样，并对内脏和SC脂肪进行超声测量。主要结果(S)：信使核糖核酸评估脂肪组织样本中的脂联素和瘦素；血清测量脂联素、瘦素、血糖、胰岛素和激素水平；超声波测量脂肪数量。对PCOS患者与对照组进行相关分析和比较。结果(S)：与对照组相比，PCOS患者存在更多的胰岛素抵抗，血清瘦素水平相近，但血清脂联素水平降低。当对照组妇女按体重指数(BMI)分为四分位数时，瘦素和脂联素的信使RNA表达随着BMI的增加而降低。脂联素和瘦素在PCOS患者中的表达显著降低；在体重匹配的患者和对照组中，SC组织中的瘦素和脂联素的表达显著降低，而脂联素在PCOS患者的大网膜组织中的表达显著降低。在对照妇女中，SC组织中的表达高于内脏组织。PCOS患者的内脏脂肪和SC脂肪质量与脂联素和瘦素的表达呈显著负相关。PCOS患者血清脂联素与内脏脂联素表达呈显著正相关(r=0.6 4)，SC脂联素表达与定量胰岛素敏感性检查指数呈正相关(r=0.43)。结论：脂肪组织中脂肪细胞因子的表达随着脂肪量的增加而下调，尤其是脂联素的表达在PCOS患者中尤为明显。胰岛素抵抗很可能是导致这些结果的一个因素。
Objective: To assess message expression of adiponectin and leptin in visceral and SC fat in women with polycystic ovary syndrome (PCOS) and in control women.Design: Prospective clinical trial.Setting: Academic medical. centers in Mexico City, Mexico and New York, New York.Patient(s): Women with PCOS and control women.Intervention(s): Surgical biopsies of visceral (omental) and subcutaneous (SC) adipose tissue, fasting blood samples, and ultrasound measurements of visceral and SC fat.Main Outcome Measure(s): Messenger RNA assessment of adiponectin and leptin in adipose tissue samples; serum measurements of adiponectin, leptin, glucose, insulin, and hormone levels; measurements of fat quantity by ultrasound. Correlative analyses as well as comparisons, between women with PCOS and control women were performed.Result(s): Confirming previous data, women with PCOS had more insulin resistance, similar serum leptin, but lower serum adiponectin compared with control women. When control women were divided into quartiles by body mass index (BMI), messenger RNA expression of leptin and adiponectin decreased with increasing BMI. Adiponectin and leptin expression was significantly lower in women with PCOS; in weight-matched patients and control women, leptin and adiponectin expression was statistically significantly lower in SC tissue, and adiponectin expression was statistically significantly lower in omental tissue in women with PCOS. In control women, there was greater expression in SC tissue compared with in visceral tissue. There were significant negative correlations between visceral and SC fat mass by both ultrasound as well as adiponectin and leptin expression in Women with PCOS. Serum adiponectin correlated statistically significantly with visceral adiponectin expression (r = 0.64) in women with PCOS, and there was a statistically significant correlation between SC adiponectin expression and the Quantitative Insulin-Sensitivity Check Index as a marker of insulin resistance (r = 0.43).Conclusion(s): Adipocytokine expression in fat tissue appears to be down-regulated by an increased fat mass; this is particularly evident in the case of adiponectin expression in women with PCOS. It is probable that insulin resistance is a factor that may contribute, in part, to these findings.