Effect of regeneration of liquid silk fibroin on its structure and characterization
Effect of regeneration of liquid silk fibroin on its structure and characterization
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DOI：
10.1039/c2sm26945g
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发表时间：
2013-01-01
期刊：
影响因子：
3.4
通讯作者：
Zhang, Yu-Qing
中科院分区：
文献类型：
--
作者：
Wang, Hai-Yan;Zhang, Yu-Qing
以家蚕蚕茧丝为原料，将其制成脱胶丝、液态丝素和再生丝素膜3种试验材料。研究了蚕丝脱胶溶纤系统（SD-FDS）对丝素蛋白分子结构和性能的影响。Na 2CO 3溶液脱胶对丝素纤维的影响最大，导致热稳定性和结晶度明显降低，纤维拉伸性能仅为尿素缓冲液脱胶的一半。适度脱胶溶液为尿素缓冲液，然后是强碱性电解水（SAEW）。SDS-PAGE显示，经80 ℃ 8.0M尿素脱胶，25 ℃ 9.3M LiBr溶解的丝素蛋白与体内天然丝素蛋白相似，但在Na_2CO_3溶液中煮沸会导致丝素蛋白肽链严重断裂。肽链的断裂程度与液态丝素蛋白的贮存时间呈正相关。差示扫描量热分析表明丝素蛋白肽链越完整，热分解温度越高。在研究过程中经常忽略丝蛋白的再生条件，而我们的研究表明，不同的蚕丝脱胶和纤维溶解体系会产生不同的影响。本试验结果有助于我们更深入地了解蚕丝脱胶和纤维溶解对丝素蛋白分子结构和性能的影响，为筛选和利用丝素蛋白作为先进的功能生物材料，如医用组织工程材料提供重要的实验数据。
Silk derived from cocoons of the female silkworm (Bombyx mori) was made into three kinds of test material, degummed fiber, liquid fibroin and regenerated silk fibroin membrane (RSFM). The effect of the silk degumming and fiber dissolution system (SD-FDS) on the molecular structure and properties of silk fibroin were studied in detail. Degumming with Na2CO3 solution had the greatest impact on silk fibroin fiber, resulting in significant reduction of the thermal stability and crystallinity, and the fiber tensile property was only half of that degummed with urea buffer. The moderate degumming solution was urea buffer, followed by strongly alkaline electrolyzed water (SAEW). SDS-PAGE showed that the silk fibroin degummed with 8.0 M urea at 80 degrees C and dissolved in 9.3 M LiBr at 25 degrees C was similar to the natural silk fibroin in vivo, but boiling in Na2CO3 solution leads to serious breakage of the silk fibroin peptide chain. The degree of breakage of the peptide chain is positively correlated with the storage time of liquid silk fibroin. DSC showed the more nearly intact the silk fibroin peptide chain, the higher the thermal decomposition temperature. The regenerated condition of the silk protein is often overlooked during the research process, while our study showed that different silk degumming and fiber dissolution systems will have different influences. The results of this test help us gain a deeper understanding of the effect of silk degumming and fiber dissolution on the molecular structure and properties of silk fibroin and provide important experimental data for screening and using silk protein as advanced functional biomaterials, such as medical tissue engineering materials.