In vitro evaluation of Ag-containing calcium phosphates: Effectiveness of Ag-incorporated β-tricalcium phosphate
In vitro evaluation of Ag-containing calcium phosphates: Effectiveness of Ag-incorporated β-tricalcium phosphate
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DOI：
10.1016/j.msec.2017.02.059
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发表时间：
2017-06-01
影响因子：
7.9
通讯作者：
Narushima, Takayuki
中科院分区：
文献类型：
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作者：
Gokcekaya, Ozkan;Ueda, Kyosuke;Narushima, Takayuki
开发具有抗菌活性、无细胞毒性的生物陶瓷将有利于预防种植体相关感染。这项研究的目的是利用与磷酸钙(CAPS)金属形式结合或共存的银(Ag)的抗菌性能。考察了不同相组成的羟基磷灰石(HA)和β-磷酸三钙(β-TCP)的体外溶出行为、抗菌活性和细胞毒性。含银CAPS的抗菌活性取决于CAP的主相、银的化学状态和银的掺入量。与与金属银共存的羟基磷灰石相比，载银β-磷酸三钙通过持续溶解的方式持续释放银离子获得了更好的抗菌活性。含银CAPS对V79成纤维细胞无毒性作用。因此，这些结果表明，就长期应用而言，掺银的β-磷酸三钙在预防感染方面是有效的。(C)2017爱思唯尔B.V.保留所有权利。
Development of bioceramics with antibacterial activity and without cytotoxicity would be beneficial for preventing infection associated with implants. This study aimed to capitalize on the antibacterial properties of silver (Ag) incorporated in or coexisting in metallic form with calcium phosphates (CaPs). The in vitro dissolution behavior, antibacterial activity, and cytotoxicity of Ag-containing CaPs with different phase fractions of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) were evaluated. The antibacterial activity of Ag-containing CaPs depended on the main phase of CaP), the chemical state of Ag, and the amount of incorporated Ag. Superior antibacterial activity was obtained from sustained release of Ag ions through continuous dissolution of Ag-incorporated beta-TCP compared to that obtained for HA coexisting with metallic Ag particles. Ag-containing CaPs did not exhibit any toxic effect on V79 fibroblasts. Thus, these results demonstrated the effectiveness of Ag-incorporated beta-TCP in preventing infection, with respect to long-term applications. (C) 2017 Elsevier B.V. All rights reserved.