CADMIUM IN-VIVO CAUSES DISRUPTION OF TIGHT JUNCTION-ASSOCIATED MICROFILAMENTS IN RAT SERTOLI CELLS
CADMIUM IN-VIVO CAUSES DISRUPTION OF TIGHT JUNCTION-ASSOCIATED MICROFILAMENTS IN RAT SERTOLI CELLS
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DOI：
10.1095/biolreprod49.4.840
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发表时间：
1993-10-01
影响因子：
3.6
通讯作者：
WELSH, MJ
中科院分区：
文献类型：
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作者：
HEW, KW;HEATH, GL;WELSH, MJ
10周龄雄性Sprague-Dawley大鼠腹腔注射氯化镉溶液，单次剂量为0或1.0 mg/kg BW。在注射后4、24、48和72 h，收集动物睾丸，脱套，并固定在10%福尔马林中。分离单个生精小管，并通过在体视显微镜下使用透照法确定生精上皮的周期阶段。将小管用罗丹明-鬼笔环肽染色，固定在载玻片上，并通过共聚焦显微镜检查。该镉剂量未引起睾丸中可见的血管病变。镉处理导致支持细胞基底区微丝产生的图案发生变化。与对照组动物的曲细精管相比，观察到的基底支持细胞微丝的变化包括微丝束的断裂。这种明显的病变，第一次观察到在第VIII至XI阶段在镉暴露后24小时。随着暴露后时间的增加，一个阶段内的病变发生的严重程度增加，生精上皮细胞周期的后期阶段也受到影响。暴露后48小时，在VIII至XIII/XIV阶段观察到微丝束的解体。暴露后72小时，从第VIII阶段到第II/III阶段观察到微丝束严重断裂。在第VIII阶段之前的几个阶段中的微丝束保持不受影响。管周细胞微丝无明显变化。暴露后4 h，睾丸支持细胞基底区的微丝束或小管周细胞的微丝组织无变化。我们的结论是，一个单一的氯化镉剂量为1毫克/公斤的结果在破坏的基础支持细胞微丝束在大鼠生精上皮，镉的行动是细胞特异性和阶段特异性。
Ten-week-old male Sprague-Dawley rats were injected i.p. with cadmium chloride solution in a single dose of 0 or 1.0 mg/kg BW. At 4, 24, 48, and 72 h after injection, testes of the animals were collected, detunicated, and fixed in 10% formalin. Individual seminiferous tubules were isolated and the stages of the cycle of the seminiferous epithelium were determined through use of transillumination under a stereomicroscope. The tubules were stained with rhodamine-phalloidin, mounted on glass slides, and examined via a confocal microscope. This cadmium dose did not cause visible vascular lesion in the testes. The cadmium treatment resulted in changes in the pattern produced by microfilaments in the basal region of Sertoli cells. The observed change in basal Sertoli cell microfilaments consisted of fragmentation of the microfilament bundles as compared to those in seminiferous tubules from control animals. This apparent lesion was first observed in stages VIII through XI at 24 h after the cadmium exposure. As the time after exposure increased, the lesion within a stage occurred with increasing severity, and later stages of the cycle of the seminiferous epithelium were also affected. At 48 h after exposure, disorganization of microfilament bundles was seen in stages VIII through XIII/XIV. At 72 h after exposure, severe fragmentation of microfilament bundles was observed from stage VIII through stages II/III. The microfilament bundles in several stages prior to stage VIII remained unaffected. No change was observed in the microfilaments of peritubular cells. At 4 h after exposure, testes showed no change in the organization of microfilament bundles at the basal region of Sertoli cells or microfilaments in the peritubular cells. We conclude that a single cadmium chloride dose of 1 mg/kg results in the disruption of basal Sertoli cell microfilament bundles in the rat seminiferous epithelium, and that the action of cadmium is cell-specific and stage-specific.