Molecular epidemiological characterization of poultry red mite, Dermanyssus gallinae, in Japan.
Molecular epidemiological characterization of poultry red mite, Dermanyssus gallinae, in Japan.
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日本家禽红螨 Dermanyssus gallinae 的分子流行病学特征。
DOI：
10.1292/jvms.15-0203
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Yamaguchi T
中科院分区：
文献类型：
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作者：
Chu TT;Murano T;Uno Y;Usui T;Yamaguchi T
鸡皮螨（Dermanyssus gallinae），即家禽红螨，是一种专性吸血体外寄生虫。一些国家已经对鸡蝇的遗传多样性进行了检查，但迄今为止尚未在亚洲国家进行过检查。在这里，我们对从日本 40 个县收集的 239 个螨虫样本中的部分线粒体细胞色素氧化酶亚基 I (COI) 和 16S rRNA 基因以及核内转录间隔区 (ITS) 区域进行了测序。 COI和16S rRNA核苷酸序列分别分为28和26个单倍型。在系统发育树中，单倍型聚类成2个单倍群，对应于之前报道的单倍群A和B。单倍群 A 和 B 分别进一步细分为亚单倍群 AJ1 和 AJ2，以及 BJ1 和 BJ2。在这两棵树中，AJ1和BJ2中的单倍型序列与其他国家报道的单倍型序列相对较远，而AJ2和BJ1中的一些序列与欧洲的序列相同。此外，ITS序列被分为两个序列，两个序列都与欧洲国家发现的序列密切相关。这些发现表明鸡蛔虫有可能进行国际海外传播。
Dermanyssus gallinae, the poultry red mite, is an obligatory blood-sucking ectoparasite. The genetic diversity of D. gallinae has been examined in some countries, but so far not in Asian countries. Here, we sequenced a part of the mitochondrial cytochrome oxidase subunit I (COI) and16S rRNA genes and nuclear internal transcribed spacers (ITS) region in 239 mite samples collected from 40 prefectures throughout Japan. The COI and 16S rRNA nucleotide sequences were classified into 28 and 26 haplotypes, respectively. In phylogenetic trees, the haplotypes clustered into 2 haplogroups corresponding to haplogroups A and B, which were previously reported. Haplogroups A and B were further subdivided into sub-haplogroups AJ1 and AJ2, and BJ1 and BJ2, respectively. In both trees, the sequences of haplotypes in AJ1 and BJ2 were relatively distant from those reported in other countries, while some sequences in AJ2 and BJ1 were identical to those in Europe. In addition, the ITS sequences were classified into two sequences, and both sequences were closely related to the sequences found in European countries. These findings indicate a possibility of international oversea transmission of D. gallinae.