miR-372 suppresses tumour proliferation and invasion by targeting IGF2BP1 in renal cell carcinoma
miR-372 suppresses tumour proliferation and invasion by targeting IGF2BP1 in renal cell carcinoma
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miR-372通过靶向IGF2BP1抑制肾细胞癌中的肿瘤增殖和侵袭
DOI：
10.1111/cpr.12207
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发表时间：
2015-10-01
影响因子：
8.5
通讯作者：
Li, Yong
中科院分区：
文献类型：
--
作者：
Huang, Xuan;Huang, Mingjie;Li, Yong
目标MicroRNA (miRNA) 是内源性小非编码 RNA，可调节蛋白质和 mRNA 的降解或翻译抑制。迄今为止，miR-372在肾细胞癌中的作用仍不清楚；在本研究中，我们的目的是揭示其在该肿瘤中的功能重要性。材料和方法进行qRT-PCR来测量肾细胞癌细胞系和组织中miR-372的表达水平。进行 CCK-8 和侵袭测定来测量其功能作用。通过荧光素酶测定、qRT-PCR 和蛋白质印迹来发现 miR-372s 靶基因。结果我们证明 miRNA-372 在肾细胞癌细胞系和组织标本中下调；其过度表达抑制细胞增殖和侵袭。此外，我们发现 miRNA-372 通过直接与其 3-UTR 上的假定结合位点相互作用来抑制胰岛素样生长因子 2 mRNA 结合蛋白 1 (IGF2BP1) 的表达。此外，IGF2BP1的异位表达显着逆转了miR-372过表达引起的细胞增殖和侵袭的抑制。结论我们的数据表明，miR-372似乎通过抑制IGF2BP1表达而在肾细胞癌进展中发挥抑癌作用。
ObjectivesMicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate proteins and mRNAs for degradation or translational suppression. Up to now, the role of miR-372 in renal cell carcinoma has remained unknown; in this study, we have aimed to reveal its functional importance in this tumour.Materials and methodsqRT-PCR was performed to measure expression levels of miR-372 in renal cell carcinoma cell lines and tissues. CCK-8 and an invasion assay were performed to measure its functional role. Luciferase assays, qRT-PCR and western blotting were performed to discover miR-372s target gene.ResultsWe demonstrated that miRNA-372 was down-regulated in renal cell carcinoma cell lines and tissue specimens; its over-expression inhibited cell proliferation and invasion. Moreover, we showed that miRNA-372 repressed insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) expression by directly interacting with its putative binding site at the 3-UTR. Furthermore, ectopic expression of IGF2BP1 significantly reversed suppression of cell proliferation and invasion caused by miR-372 over-expression.ConclusionsOur data indicated that miR-372 seemed to function as a tumour suppressor in renal cell carcinoma progression by inhibiting the IGF2BP1 expression.