3D optical imaging of multiple SERS nanotags in cells
3D optical imaging of multiple SERS nanotags in cells
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DOI：
10.1039/c3sc51437d
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发表时间：
2013-01-01
期刊：
影响因子：
8.4
通讯作者：
Graham, Duncan
中科院分区：
文献类型：
--
作者：
McAughtrie, Sarah;Lau, Katherine;Graham, Duncan
最近的研究清楚地表明，拉曼光谱和表面增强拉曼散射 (SERS) 光谱是信息丰富的非破坏性技术，可用于监测细胞内的细微变化。然而，尽管这些技术在细胞研究中得到了证明和复杂的应用，但仍然缺乏随附的 3D 图像。在此，我们首次展示了 3D 拉曼和 SERS 成像相结合，用于同时确认 SERS 纳米标签的细胞包涵体和多组分检测。我们还报告了通过多变量分析方法对细胞核进行 3D 解析。 3D 成像对于了解患病细胞和健康细胞及组织之间的结构变化至关重要。它还将提供细胞摄取的非破坏性明确证据，同时确认 SERS 纳米标签到达其预期目的地。
Recent studies have clearly demonstrated that Raman and surface enhanced Raman scattering (SERS) spectroscopies are information rich, non-destructive techniques for the monitoring of subtle intracellular changes. However, despite the demonstrated and sophisticated applications of these techniques in cell studies there still remains a lack of accompanying 3D images. Herein we demonstrate for the first time combined 3D Raman and SERS imaging for the simultaneous confirmation of the cellular inclusion and multiple component detection of SERS nanotags. We also report on the 3D elucidation of the cell nuclei by multivariate analysis methods. Imaging in 3D will be critical to understanding architectural changes between diseased and healthy cells, and tissues. It will also provide non-destructive definitive proof of cellular uptake whilst simultaneously confirming targeting of SERS nanotags to their intended destinations.