Nanodiscs and mass spectrometry: Making membranes fly
Nanodiscs and mass spectrometry: Making membranes fly
复制标题
DOI：
10.1016/j.ijms.2020.116436
复制
发表时间：
2020-12-01
影响因子：
1.8
通讯作者：
Marty, Michael T.
中科院分区：
文献类型：
--
作者：
Marty, Michael T.
细胞被保护性的脂质双层膜包围，双层膜中的膜蛋白控制着化学物质、信息和能量穿过这一屏障的流动。许多治疗剂靶向膜蛋白，有些直接靶向脂质膜本身。然而，由于生物膜的异质性和不溶性，生物膜内的相互作用研究具有挑战性。质谱（MS）已成为研究膜蛋白，特别是膜蛋白如何与周围脂质环境相互作用的强大技术。虽然洗涤剂胶束是最常见的膜模拟物，纳米盘正在成为MS的一个有前途的平台。纳米盘，由两个支架蛋白包围的纳米级脂质双层，提供了一个可控制的脂质双层用于溶解膜蛋白。这个年轻的科学家的观点集中在完整的纳米盘的原生MS，并强调了通过使膜飞行，包括研究膜蛋白-脂质相互作用和探索脆弱的跨膜肽复合物的特异性启用的独特实验。它还将探讨当前的挑战和未来的前景与MS接口nanodisks.（c）2020 Elsevier B. V.保留所有权利。
Cells are surrounded by a protective lipid bilayer membrane, and membrane proteins in the bilayer control the flow of chemicals, information, and energy across this barrier. Many therapeutics target membrane proteins, and some directly target the lipid membrane itself. However, interactions within biological membranes are challenging to study due to their heterogeneity and insolubility. Mass spectrometry (MS) has become a powerful technique for studying membrane proteins, especially how membrane proteins interact with their surrounding lipid environment. Although detergent micelles are the most common membrane mimetic, nanodiscs are emerging as a promising platform for MS. Nanodiscs, nanoscale lipid bilayers encircled by two scaffold proteins, provide a controllable lipid bilayer for solubilizing membrane proteins. This Young Scientist Perspective focuses on native MS of intact nanodiscs and highlights the unique experiments enabled by making membranes fly, including studying membrane protein-lipid interactions and exploring the specificity of fragile transmembrane peptide complexes. It will also explore current challenges and future perspectives for interfacing nanodiscs with MS. (c) 2020 Elsevier B.V. All rights reserved.