Use of bomb pulse carbon-14 to age senile plaques and neurofibrillary tangles in Alzheimer's disease
Use of bomb pulse carbon-14 to age senile plaques and neurofibrillary tangles in Alzheimer's disease
复制标题
DOI：
10.1016/s0197-4580(01)00281-0
复制
发表时间：
2002-03-01
影响因子：
4.2
通讯作者：
Markesbery, WR
中科院分区：
文献类型：
--
作者：
Lovell, MA;Robertson, JD;Markesbery, WR
The time course of formation of neurofibrillary tangles (NFT) and senile plaques (SP) in Alzheimer's disease (AD) brain is unknown. Above ground nuclear weapons testing in the late 1950s and early 1960s led to significantly increased levels of C-14 in the atmosphere and carbon cycle. Because the amyloid beta peptide of SP and paired helical filaments of NFT, once formed, are relatively resistant to degradation, C-14 levels observed in SP and NFT should reflect their year of formation. The purpose of this study was to develop a method to determine whether C-14 levels could be used to define NFT and SP ages. Using accelerator mass spectrometry to measure bomb-pulse C-14 levels, we determined the average age of formation of isolated SP and NFT fractions in bulk brain samples of 6 AD subjects. Although preliminary, the results demonstrate that it is possible to use bomb pulse C-14 to determine the average year of formation of NFT and SP in the brain in AD. In addition, the data show that these structures, once formed, have a much slower carbon turnover rate than normal brain and are not in a formation/enzymatic degradation equilibrium. (C) 2002 Elsevier Science Inc. All rights reserved.
