TECHNICAL CONSIDERATIONS ON USE OF HORSERADISH-PEROXIDASE AS A NEURONAL MARKER
TECHNICAL CONSIDERATIONS ON USE OF HORSERADISH-PEROXIDASE AS A NEURONAL MARKER
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DOI：
10.1016/0306-4522(77)90074-4
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发表时间：
1977-01-01
期刊：
影响因子：
3.3
通讯作者：
ADAMS, JC
中科院分区：
文献类型：
--
作者：
ADAMS, JC
描述了提高辣根过氧化物酶逆行标记神经元的可靠性和灵敏度的技术。通过在轴突被切断的地方注射过氧化物酶来增加标记。建议专门使用戊二醛作为固定剂并缩短用冷冻保护剂渗透组织的时间，以减少注射的过氧化物酶的降解。通过没食子蓝素复染、使用相差光学器件以及通过在氯化钴中对组织进行预孵育处理来促进反应产物的定位。考虑了二氨基联苯胺的使用及其安全处置。
Techniques are described for increasing the reliability and sensitivity of retrograde labeling of neurons with horseradish peroxidase. Labeling was increased by injecting peroxidase where axons are severed. Exclusive use of glutaraldehyde as a fixative and shortening the time used to infiltrate tissue with cryoprotectives were recommended to reduce degradation of injected peroxidase. Localization of the reaction product was facilitated by gallocyanin counterstaining, by use of phase-contrast optics and by preincubation treatment of tissue in cobalt chloride. The use of diaminobenzidine and its safe disposal were considered.